Translational Research
Volume 155, Issue 3 , Pages 142-147, March 2010

Resistin gene promoter region polymorphism and the risk of hypertrophic cardiomyopathy in patients

  • Sabir Hussain

      Affiliations

    • Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan
  • ,
  • Muhammad Asghar

      Affiliations

    • Siddique Sadiq Memorial Trust Cardiac Institute, Gujranwala, Pakistan
  • ,
  • Qamar Javed

      Affiliations

    • Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan
    • Corresponding Author InformationReprint requests: Dr. Qamar Javed, Department of Biochemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan

Received 9 June 2009; received in revised form 8 October 2009; accepted 12 October 2009. published online 06 November 2009.

Resistin, a novel cytokine, is associated with an inflammatory process and is suggested to induce hypertrophy in rat cardiomyocytes. Resistin gene expression has not been investigated in patients with hypertrophic cardiomyopathy (HCM). This study investigates resistin levels in HCM patients and healthy controls and the molecular basis for the regulation of the resistin gene (RETN) in a Pakistani population. Patients with HCM (n = 105) and healthy individuals (n = 110) were enrolled in this investigation. Serum resistin levels were determined by enzyme-linked immunosorbent assay (ELISA). RETN genotyping was performed by polymerase chain reaction (PCR) and DNA sequencing. Our data showed a statistically significant increase in resistin levels from HCM patients compared with healthy subjects (6.3 ± 2.7 ng/mL in patients vs 3.4 ± 2.1 ng/mL in controls, P < 0.0001). The RETN –420 C > G polymorphism was significantly high in patients with HCM compared with the control group (P < 0.001). There was a significant difference between the C and G alleles from HCM cases and controls (odds ratio [OR] = 3.54, 95% confidence interval [CI] = 2.36–5.30, P < 0.0001). Logistic-regression analysis showed that the increased resistin levels, and the RETN–420 C > G polymorphism were significantly associated with HCM. Our data suggest that the elevated resistin levels and the RETN –420 C > G polymorphism may be associated with cardiac hypertrophy in the study population.

Abbreviations: ECG, electrocardiography, EDTA, ethylenediamine tetra acetic acid, HCM, hypertrophic cardiomyopathy, HDL-C, high-density cholesterol, IRB, Institutional Review Board, LDL-C, low-density cholesterol, PCR, polymerase chain reaction, RETN, resistin gene, SNPs, single-nucleotide polymorphisms, TC, total cholesterol, TG, triglycerides

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 Supported by a research grant from the Higher Education Commission, Pakistan, for a Ph.D. study.

PII: S1931-5244(09)00302-8

doi:10.1016/j.trsl.2009.10.002

Translational Research
Volume 155, Issue 3 , Pages 142-147, March 2010