Research Article| Volume 41, ISSUE 5, P825-828, May 1953

The determination of serum acid phosphatase activity

      This paper is only available as a PDF. To read, Please Download here.


      A simple, reproducible method for the determination of serum acid phosphatase activity has been described. The measurement of enzyme activity may be carried out with as little as 0.05 ml. of serum. It is tentatively proposed that serum acid phosphatase levels above 1.50 micromole units be considered pathologic.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Kaplan A.
        • Narahara A.
        The Determination of Serum Alkaline Phosphatase Activity.
        J. Lab. & Clin. Med. 1953; 41 (this issue): 819
        • Gomori G.
        Determination of Phenol in Biologic Material.
        J. Lab. & Clin. Med. 1949; 34: 275-281
        • Massart L.
        Enzyme Inhibition.
        in: The Enzymes. Vol. 1. Academic Press, Inc.,, New York1950: 307-342 (Part 1)
        • Roche J.
        in: The Enzymes. Vol. 1. Academic Press, Inc.,, New York1950: 473-510 (Part 1)
        • Munemura S.
        Influence of Electrolytes on Phosphomonoesterase and Pyrophosphatase.
        J. Biochem. (Japan). 1933; 17: 343-366
        • Belfanti S.
        • Contardi A.
        • Ercoli A.
        LX. Studies on the Phosphatases. The Influence of Some Electrolytes on the Phosphatases of Animal Tissue. Phosphatases of the Liver, Kidney, Serum and Bones of the Rabbit.
        Biochem. J. 1935; 29: 517-527
        • Belfanti S.
        • Contardi A.
        • Ercoli A.
        XCIX. Researches on the Phosphatases. II. Inactivation and Reactivation of the Phosphatases of Animal Organs.
        Biochem. J. 1935; 29: 842
        • Sullivan T.J.
        • Gutman E.B.
        • Gutman A.B.
        Theory and Application of the Serum “Acid” Phosphatase Determination in Metastasizing Prostatic Carcinoma; Early Effects of Castration.
        J. Urol. 1942; 48: 426-458
        • Gutman E.B.
        • Gutman A.B.
        Erythrocyte Phosphatase Activity in Hemolyzed Sera and Estimation of Serum “Acid” Phosphatases.
        in: Proc. Soc. Exper. Biol. & Med. 47. 1941: 513-515