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Abstract
Studies were undertaken upon the preparation of purified and stable influenza virus
for use in the serological diagnosis of influenza. Neither crude allantoic fluid or
virus suspensions prepared following the methods of other workers had proved satisfactory
because of the formation of precipitates with accompanying loss of virus activity
upon storage.
Methanol was the most satisfactory precipitating agent. Each of three strains of influenza
virus, PR-8, FM-1, and LEE, apparently have a characteristic of being precipitated
optimally by methanol in final concentrations of 26 per cent for PR-8, 19 per cent
for FM-1, and 27 per cent for LEE.
Alteration of the pH of crude allantoic fluid from 8.25 to 7.0 prior to precipitation
with varying alcohol concentrations had no effect upon the yield of virus.
The buffer of choice for eluting and suspending the precipitated influenza virus was
found to be a 0.1M mono- and disodium phosphate buffer, pH 7.0. Virus suspensions
in this buffer, when stored at 4 ° C., have remained clear and maintained a high chick
cell agglutinating activity for periods in excess of twelve months.
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References
- The Preparation and Properties of Influenza Virus Vaccines Concentrated and Purified by Differential Centrifugation.J. Exper. Med. 1945; 81: 193-217
- The Purification and Concentration of Influenza Virus by Means of Alcohol Precipitation.J. Immunol. 1947; 56: 149-164
- Methanol Precipitation of Influenza Virus.Science. 1950; 112: 459-460
- A Method for the Titration of Influenza Hemagglutinins and Influenza Antibodies with the Aid of a Photoelectric Densitometer.J. Immunol. 1942; 45: 273-285
- Concentration of Influenza Virus (PR-8 strain) by a Cation Exchange Risen.in: Proc. Soc. Exper. Biol. & Med. 80. 1952: 27-29
- The Efficiency of Different Sharples Centrifuge Bowls in the Concentration of Tobacco Mosaic and Influenza Viruses.J. Immunol. 1946; 53: 179-190
Article info
Publication history
Received:
September 10,
1952
Footnotes
☆Consultants were Clayton G. Loosli, M.D., University of Chicago, and Thomas G. Ward, M.D., The Johns Hopkins School of Public Health and Hygiene.
☆☆This study was done in connection with Research Project NM 005 051.14, The Bureau of Medicine and Surgery, Navy Department.
★The opinions and assertions contained in this report are those of the authors, and are not to be construed as official or as reflecting the views of the Navy Department or of the Naval service at large.
Identification
Copyright
© 1953 Published by Elsevier Inc.
