Research Article| Volume 41, ISSUE 2, P290-295, February 1953

Purification and stabilization of influenza virus antigens for use in hemagglutination-inhibition or complement fixation antibody titrations

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      Studies were undertaken upon the preparation of purified and stable influenza virus for use in the serological diagnosis of influenza. Neither crude allantoic fluid or virus suspensions prepared following the methods of other workers had proved satisfactory because of the formation of precipitates with accompanying loss of virus activity upon storage.
      Methanol was the most satisfactory precipitating agent. Each of three strains of influenza virus, PR-8, FM-1, and LEE, apparently have a characteristic of being precipitated optimally by methanol in final concentrations of 26 per cent for PR-8, 19 per cent for FM-1, and 27 per cent for LEE.
      Alteration of the pH of crude allantoic fluid from 8.25 to 7.0 prior to precipitation with varying alcohol concentrations had no effect upon the yield of virus.
      The buffer of choice for eluting and suspending the precipitated influenza virus was found to be a 0.1M mono- and disodium phosphate buffer, pH 7.0. Virus suspensions in this buffer, when stored at 4 ° C., have remained clear and maintained a high chick cell agglutinating activity for periods in excess of twelve months.
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