Research Article| Volume 41, ISSUE 2, P296-299, February 1953

Ninhydrin-schiff and alloxan-schiff staining

A new histochemical staining method for protein
      This paper is only available as a PDF. To read, Please Download here.


      A new histochemical test for protein and peptide, based on the liberation aldehyde groups by ninhydrin or alloxan oxidation, and subsequent demonstration of aldehyde by reduced fuchsin, has been described. Experiments of enzymatic digestion and spot test showed the specificity of this reaction. It was compared with three present histochemical techniques for protein, and proved to be preferable in many respects.
      This method, named A.S. or N.S. stain, gives a distinct color reaction, according to the distribution of protein in tissues.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Ries E.
        Grundriss der Histophysiologie.
        Akademische Verlagsgesellachaft M.B.H, Leipzig1938
        • Berg W.
        Ueber Anwendung der Ninhydrinreaktion auf mikroskopische Präparate zum Nachweiss niederer Eiweisskörper.
        Pflügers Arch. 1922; 195: 543
        • Ruhemann J.
        Chem. Soc. 1911; 99: 792
        • Ruhemann J.
        Chem. Soc. 1911; 99: 1486
        • Abderhalden R.
        Z. Physiol. Chem. 1938; 252: 81
        • Akahori S.
        Amino Acid and Protein (In Japanese).
        (3 and 4, referred from) Kyoritsu-Shuppansha, Tokyo1944
        • Shibatani A.
        Detection of Lower Proteins in Mammarian Tissues by Means of Histochemieal Ninhydrin Reaction, with Special Reference to Their Relation with Ribonucleic Acid in the Cytoplasm.
        Physiol. & Ecol. 1948; 2: 24
        • Feulgen R.
        u. Rosenbeck H. Mikroskopisch-chemischer Nachweiss einer Nucleinsäure vom Typus der Thymonucleinsäure und die darauf beruhende elekive Färbung von Zellkernen in migroskopischen Präparaten.
        Z. Physiol. Chem. 1924; 135: 203
        • Hotchkiss R.D.
        A Microchemical Reaction Resulting in the Staining of Polysaccharide Structures in Fixed Tissue Preparations.
        Arch. Biochem. 1948; 16: 131
        • Sherry S.
        • Goeller J.P.
        The Extent of Enzymatic Degradation of Desoxyribonucleic Acid (DNA) in Purulent Exudates by Streptodornase.
        J. Clin. Investigation. 1950; 29: 1588