This paper is only available as a PDF. To read, Please Download here.
Abstract
A new histochemical test for protein and peptide, based on the liberation aldehyde
groups by ninhydrin or alloxan oxidation, and subsequent demonstration of aldehyde
by reduced fuchsin, has been described. Experiments of enzymatic digestion and spot
test showed the specificity of this reaction. It was compared with three present histochemical
techniques for protein, and proved to be preferable in many respects.
This method, named A.S. or N.S. stain, gives a distinct color reaction, according
to the distribution of protein in tissues.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Translational ResearchAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Grundriss der Histophysiologie.Akademische Verlagsgesellachaft M.B.H, Leipzig1938
- Ueber Anwendung der Ninhydrinreaktion auf mikroskopische Präparate zum Nachweiss niederer Eiweisskörper.Pflügers Arch. 1922; 195: 543
- Chem. Soc. 1911; 99: 792
- Chem. Soc. 1911; 99: 1486
- Z. Physiol. Chem. 1938; 252: 81
- Amino Acid and Protein (In Japanese).(3 and 4, referred from) Kyoritsu-Shuppansha, Tokyo1944
- Detection of Lower Proteins in Mammarian Tissues by Means of Histochemieal Ninhydrin Reaction, with Special Reference to Their Relation with Ribonucleic Acid in the Cytoplasm.Physiol. & Ecol. 1948; 2: 24
- u. Rosenbeck H. Mikroskopisch-chemischer Nachweiss einer Nucleinsäure vom Typus der Thymonucleinsäure und die darauf beruhende elekive Färbung von Zellkernen in migroskopischen Präparaten.Z. Physiol. Chem. 1924; 135: 203
- A Microchemical Reaction Resulting in the Staining of Polysaccharide Structures in Fixed Tissue Preparations.Arch. Biochem. 1948; 16: 131
- The Extent of Enzymatic Degradation of Desoxyribonucleic Acid (DNA) in Purulent Exudates by Streptodornase.J. Clin. Investigation. 1950; 29: 1588
Article info
Publication history
Received:
June 20,
1952
Identification
Copyright
© 1953 Published by Elsevier Inc.
