Clinical and experimental| Volume 44, ISSUE 2, P185-193, August 1954

The isolation of fractions from pasteurella pestis for use in a hemagglutination test

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      Fractions have been obtained from saline extracts of acetone-killed cells of Past. pestis by (NH4)2SO4 or acid precipitation. These fractions (ESF) are adsorbed by sheep RBC and cause erythrocytes so treated to agglutinate in the presence of antiplague serum. Hemagglutination is more sensitive and more specific than bacterial agglutination for the detection of plague antibodies and may be used as an approximation of the degree of protection obtained in tests for the efficacy of immunizing-agents.
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