Research Article| Volume 63, ISSUE 1, P30-43, January 1964

Virologic studies on acute respiratory disease in young adults. II. Characteristics and serologic studies of three new rhinoviruses

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      A study of viruses associated with upper respiratory infections has yielded a number of new viruses. Seven of these new viruses representing 3 distinct serologic groups have been studied in detail. The viruses were ether resistant, acid sensitive, less than 50 mμ in size, and not inhibited by 200 μM of 2-(α-hydroxybenzyl)-benzimidazole. They were tentatively classified as RNA viruses by their lack of inhibition by 5-fluoro-2′-deoxyuridine. They were stable at 4 °C. and unstable at 56 °C. The viruses were not neutralized by antisera for the following enteroviruses: ECHO 1–25, 6′, 6″, Coxsackie A9, Coxsackie B1–5, and polioviruses types 1–3. Their growth characteristics and their acid lability distinguish them from the remainder of the Coxsackie A group. They were serologically distinct from ECHO 28 (JH-2060) and distinct from the 6 English rhinoviruses, B632, HGP, Norman, Thompson, Math Eq, and FEB and from the picornaviruses reported by the National Institutes of Health, NIH 353 1059 1734, 11757, and 33342. The 7 viruses fulfill the major criteria for etiologic agents of the “common cold.” They were recovered only from specimens obtained from students during the acute stage of natural “common colds.” There was a significant rise in antibody in every student. No significant bacteria were isolated.
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