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Abstract
Small amounts of paraffin, as particles in serum or dissolved in an organic solvent,
prevent the full development of color with cholesterol in the Lieberman-Burchard reagent,
resulting in low apparent values. Hence, the use of paraffin on stoppers or glassware
is a potential source of error in cholesterol extraction.
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References
- Cholesterol; Chemistry, Biochemistry, and Pathology.Academic Press, Inc, New York1958
- The Microtitration of Total Fatty Acids of Serum, With Notes on the Estimation of Triglyceride.J. Lipid Res. 1959; 1: 53
- A Stable Reagent for the Lieberman-Burchard Reaction.Anal. Chem. 1961; 33: 1405
Article info
Publication history
Accepted:
September 5,
1963
Received:
July 26,
1963
Footnotes
☆This study was aided by a grant from The Upjohn Company, Kalamazoo, Mich.
Identification
Copyright
© 1964 Published by Elsevier Inc.
