Research Article| Volume 63, ISSUE 1, P158-160, January 1964

Effect of paraffin on cholesterol extraction

      This paper is only available as a PDF. To read, Please Download here.


      Small amounts of paraffin, as particles in serum or dissolved in an organic solvent, prevent the full development of color with cholesterol in the Lieberman-Burchard reagent, resulting in low apparent values. Hence, the use of paraffin on stoppers or glassware is a potential source of error in cholesterol extraction.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Cook R.P.
        Cholesterol; Chemistry, Biochemistry, and Pathology.
        Academic Press, Inc, New York1958
        • Albrink M.J.
        The Microtitration of Total Fatty Acids of Serum, With Notes on the Estimation of Triglyceride.
        J. Lipid Res. 1959; 1: 53
        • Huang T.C.
        • Chen C.P.
        • Wefler V.
        • Raferty A.
        A Stable Reagent for the Lieberman-Burchard Reaction.
        Anal. Chem. 1961; 33: 1405