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Small amounts of paraffin, as particles in serum or dissolved in an organic solvent, prevent the full development of color with cholesterol in the Lieberman-Burchard reagent, resulting in low apparent values. Hence, the use of paraffin on stoppers or glassware is a potential source of error in cholesterol extraction.
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- Cholesterol; Chemistry, Biochemistry, and Pathology.Academic Press, Inc, New York1958
- The Microtitration of Total Fatty Acids of Serum, With Notes on the Estimation of Triglyceride.J. Lipid Res. 1959; 1: 53
- A Stable Reagent for the Lieberman-Burchard Reaction.Anal. Chem. 1961; 33: 1405
Accepted: September 5, 1963
Received: July 26, 1963
☆This study was aided by a grant from The Upjohn Company, Kalamazoo, Mich.
© 1964 Published by Elsevier Inc.