Advertisement
Research Article| Volume 64, ISSUE 4, P529-539, October 1964

Immunochemical characterization and serologic behavior of antibodies against red cells in infectious mononucleosis

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Sera from patients with infectious mononucleosis contain antibodies against red cells of sheep, horse, ox, and rabbit and against human red cells treated with Newcastle disease virus. These antibodies have been examined by various immunochemical techniques and all found to consist almost entirely of γ1M-globulin (19S). Cross absorption and elution experiments suggest that the rabbit cell antibody is separate from the other systems. The Newcastle agglutinin is probably also separate. Antibodies against sheep, horse, and ox cells show a well-defined pattern of cross-absorption, and it is suggested either that these antibodies are separate globulins with similar but nonidentical combining sites which react with similar but nonidentical receptors on sheep, horse, and ox red cells, or that several unrelated antibodies in the sera of patients with infectious mononucleosis react with different and unrelated antigenic determinants shared by heterologous red cells.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Paul J.R.
        • Bunnell W.W.
        The presence of Heterophile Antibodies in Infectious Mononucleosis.
        Am. J. M. Sc. 1932; 183: 90
        • Davidsohn I.
        Serologic Diagnosis of Infectious Mononucleosis.
        J. A. M. A. 1937; 108: 289
        • Eyquem A.
        Sérologie de la mononucleose infectieuse.
        Nouv. rev. franç. hemat. 1961; 1: 312
        • Bailey G.H.
        • Raffel S.
        Hemolytic Antibodies for Sheep and Ox Erythrocytes in Infectious Mononucleosis.
        J. Clin. Invest. 1935; 14: 228
        • Leyton G.B.
        Ox Cell Haemolysin in Human Sera.
        J. Clin. Path. 1952; 5: 324
        • Burnet F.M.
        • Anderson S.G.
        Agglutination of Modified Human Red Cells by Sera From Cases of Infectious Mononucleosis.
        Brit. J. Exper. Path. 1946; 27: 236
        • Pike R.M.
        • Schulze M.L.
        Agglutination in Rheumatoid Arthritis Serum of Sheep Cells Sensitized With Hemolysins and With Infectious Mononucleosis Agglutinins.
        J. Immunol. 1960; 85: 523
        • Grubb R.
        • Swahn B.
        Destruction of Some Agglutinins but Not of Others by Two Sulphydryl Compounds.
        Acta path. et microbiol. scandinav. 1958; 43: 305
        • Kunkel H.G.
        ed. 2. The Plasma Proteins. vol. 1. Academic Press, Inc, New York1960: 300
        • Fahey J.L.
        • McCoy P.H.
        • Goulian M.
        Chromatography of Serum Proteins.
        J. Clin. Invest. 1958; 37: 272
        • Muschel L.H.
        • Piper D.R.
        Enzyme Treated Red Blood Cells of Sheep in the Test for Infectious Mononucleosis.
        Am. J. Clin. Path. 1959; 32: 240
        • Springer G.S.
        • Rapaport M.J.
        Specific Release of Heterogenetic “Mononucleosis Receptor” by Influenza Viruses, Receptor Destroying Enzyme, and Plant Proteases.
        in: ed. 2. Proc. Soc. Exper. Biol. & Med. 96. 1957: 103
        • Killander J.
        • Flodin P.
        The Fractionation of Serum Proteins by Gel Filtration.
        Vox Sang. 1962; 7: 113
        • Deutsch H.F.
        • Morton J.I.
        Dissociation of Human Serum Macroglobulins.
        Science. 1957; 125: 600
        • Gleeson-White M.H.
        • Heard D.H.
        • Mynors L.S.
        • Coombs R.R.A.
        Factors Influencing the Agglutinability of Red Cells: The Demonstration of a Variation in the Susceptibility to Agglutination Exhibited by the Red Cells of Individual Oxen.
        Brit. J. Exper. Path. 1950; 31: 321
        • Swain R.H.A.
        The Nature of the Newcastle Agglutinin in Infectious Mononucleosis and Infective Hepatitis.
        J. Path. Bact. 1959; 78: 67
        • Stanley N.F.
        Studies on Listeria monocytogenes: The Role of Listeria in Infectious Mononucleosis.
        Australian J. Exper. Biol. & M. Sc. 1949; 27: 132
        • Bernstein A.
        Infectious Mononucleosis.
        Medicine. 1940; 19: 85
        • Fraser K.B.
        Antibody in Glandular Fever Sera to an Antigen Common to Streptococci and Staphylococci.
        J. Path. Bact. 1954; 67: 301
        • Campbell A.C.P.
        The Incidence of Pathogenic Staphylococci in the Throat With Special Reference to Glandular Fever.
        J. Path. Bact. 1948; 60: 157
        • Leibowitz S.
        Heterophile Antibody in Normal Adults and in Patients With Virus Hepatitis.
        Am. J. Clin. Path. 1951; 21: 201
        • Coombs R.R.A.
        • Gleeson-White M.H.
        • Hall J.G.
        Factors Influencing the Agglutinability of Red Cells. II. The Agglutination of Bovine Red Cells Previously Classified as “Inagglutinable” by the Building Up of an Antiglobulin: Globulin Lattice on the Sensitized Cells.
        Brit. J. Exper. Path. 1951; 32: 195