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A simple spectrophotometric assay for diamine oxidase (histaminase) has been modified for the measurement of this enzyme in sera. It depends upon the enzymatic conversion of diaminobutane to Δ1-pyrroline and the condensation of this product with o-amino-benzaldehyde to yield a yellow chromophore. Serum levels of diamine oxidase have been determined during normal pregnancy in forty-five women. This enzymatic activity increases sharply early in the second trimester of pregnancy and is maximal early in the third trimester. No diamine oxidase activity is detectable in sera from nonpregnant individuals. On the other hand, serum levels of monoamine oxidase, as measured by the enzymatic oxidation of benzylamine, do not change during normal pregnancy and are not different from levels obtained in nonpregnant individuals. These data are presented to indicate that sera from pregnant women contain two independent amine oxidases.
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Accepted: June 4, 1964
Received: April 27, 1964
© 1964 Published by Elsevier Inc.