Research Article| Volume 64, ISSUE 4, P540-547, October 1964

Download started.


Serum amine oxidases in pregnancy

  • Charles M. McEwen Jr.
    From the National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Department of Health, Education, and Welfare, United States Public Health Service Bethesda, Md., USA
    Search for articles by this author
      This paper is only available as a PDF. To read, Please Download here.


      A simple spectrophotometric assay for diamine oxidase (histaminase) has been modified for the measurement of this enzyme in sera. It depends upon the enzymatic conversion of diaminobutane to Δ1-pyrroline and the condensation of this product with o-amino-benzaldehyde to yield a yellow chromophore. Serum levels of diamine oxidase have been determined during normal pregnancy in forty-five women. This enzymatic activity increases sharply early in the second trimester of pregnancy and is maximal early in the third trimester. No diamine oxidase activity is detectable in sera from nonpregnant individuals. On the other hand, serum levels of monoamine oxidase, as measured by the enzymatic oxidation of benzylamine, do not change during normal pregnancy and are not different from levels obtained in nonpregnant individuals. These data are presented to indicate that sera from pregnant women contain two independent amine oxidases.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Zeller E.A.
        Oxidation of Amines.
        in: ed. 2. The Enzymes. vol. 2. Academic, Press, Inc, New York1951: 536 (part 1)
        • Tabor H.
        Metabolic Studies on Histidine, Histamine, and Related Imidazoles.
        Pharm. Rev. 1954; 6: 299
        • Zeller E.A.
        Diamine Oxidases.
        in: ed. 2. The Enzymes. vol. 8. Academic Press, Inc, New York1963: 313
        • McEwen C.M.
        • Cohen J.D.
        An Amine Oxidase in Normal Human Serum.
        J. Lab. & Clin. Med. 1963; 62: 766
      1. McEwen, C. M.: Unpublished observations.

        • Hirsch J.G.
        Spermine Oxidase: An Amine Oxidase With Specificity for Spermine and Spermidine.
        J. Exper. Med. 1953; 97: 345
        • Tabor C.W.
        • Tabor H.
        • Rosenthal S.M.
        Purification of Amine Oxidase From Beef Plasma.
        J. Biol. Chem. 1954; 208: 645
        • Blaschko H.
        The Amine Oxidases of Mammalian Blood Plasma.
        Adv. Comp. Physiol. & Biochem. 1962; 1: 67
        • Yamada H.
        • Yasunobu K.T.
        Purification, Crystallization, and Properties of Plasma Monoamine Oxidase.
        J. Biol. Chem. 1962; 237: 1511
        • Buffoni F.
        • Blaschko H.
        Benzylamine Oxidase and Histaminase: Studies of a Crystalline Preparation of the Amine Oxidase of Pig Plasma.
        Biochem. J. 1963; 89: 111 P
        • Holmstedt B.
        • Tham R.
        A Spectrophotometric Method for Determination of Diamine Oxidase Activity.
        Acta physiol. scandinav. 1959; 45: 152
        • Holmstedt B.
        • Larsson L.
        • Tham R.
        Further Studies of a Spectrophotometric Method for the Determination of Diamine Oxidase Activity.
        Biochim. et biophys. acta. 1961; 48: 182
        • Braude E.A.
        • Sondheimer F.
        Studies in Light Absorption. XI.
        J. Chem. Soc. 1955; : 3754
        • Dearden J.C.
        • Forbes W.F.
        Light Absorption Studies. XII.
        Canad. J. Chem. 1958; 36: 1362
        • Zeller E.A.
        Ueber eine einfache Farbreaktion als Schwangerschaftsnachweis.
        Schweiz. med. Wchnschr. 1941; 71: 1349
        • Ahlmark A.
        Studies on the Histaminolytic Power of Plasma With Special Reference to Pregnancy.
        Acta physiol. scandinav. 1944; 9: 1
        • Kapeller-Adler R.
        A New Volumetric Method for the Determination of Histaminase Activity in Biological Fluids.
        Biochem. J. 1951; 48: 99
        • Dodge A.C.
        A Test for Pregnancy Based on the Histaminolytic Activity of Serum.
        Am. J. Obstet. & Gynec. 1952; 63: 1213
        • Wicksell F.
        Observations on Histamine and Histaminolysis in Pregnancy.
        Acta physiol. scandinav. 1949; 17: 395
        • Swanberg H.
        Histaminase in Pregnancy.
        Acta physiol. scandinav. 1950; 23: 1
        • Tabor H.
        Diamine Oxidase.
        J. Biol. Chem. 1951; 188: 125
        • Kobayashi Y.
        Blood Levels of Diamine Oxidase as a Test for Pregnancy.
        J. Lab. & Clin. Med. 1963; 62: 699