Research Article| Volume 64, ISSUE 4, P668-674, October 1964

Determination of density distribution of red cell population

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      Density distribution of cells (DDC) can be determined by the described method, in which blood cells are separated according to their density using phthalate esters as separating liquids. Methyl phthalate and di-n-butyl phthalate are mixed in different proportions to yield a battery of fluids with increments of specific gravity of 0.004. The blood is introduced over the different separating liquids in twenty microhematocrit capillaries. After centrifugation, denser cells are separated from less dense cells by a transparent layer of the non-water miscible phthalate esters. The DDC curve thus obtained represents age population distribution as determined by tagging young red cells in vivo with Fe59 and following their maturation. The similarity of the DDC curve to the osmotic fragility curve is shown for normal blood. Various deviations between these two curves occur in certain pathological conditions. The potential diagnostic value of this method is suggested.
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        • Wintrobe M.M.
        Clinical Hematology.
        ed. 5. Lea & Febiger, 1961
        • Balentine R.
        • Burford D.D.
        Differential Density Separation of Cellular Suspension.
        Anal. Biochem. 1960; 1: 263
        • Stewart W.B.
        • Stewart J.M.
        Age as Affecting the Osmotic and Mechanical Fragility of Dog Erythrocytes Tagged With Radioactive Iron.
        J. Exper. Med. 1950; 91: 147
        • Allison A.
        • Burn G.
        Enzyme Activity as a Function of Age in the Human Erythrocyte.
        Brit. J. Haematol. 1955; 1: 291
        • Chalfin D.
        Differences Between Young and Mature Rabbit Erythrocytes.
        J. Cell. Comp. Physiol. 1956; 47: 215
        • Borun E.
        • Figueroa W.
        • Perry S.
        The Distribution of Fe59-Tagged Human Erythrocytes in Centrifuged Specimens as a Function of Cell Age.
        J. Clin. Invest. 1957; 36: 676
        • Prankerd T.A.J.
        The Aging of Red Cells.
        J. Physiol. 1958; 143: 325
        • Hoffman J.F.
        On the Relationship of Certain Erythrocyte Characteristics to Their Physiological Age.
        J. Cell Comp. Physiol. 1958; 51: 415
        • Marks P.A.
        • Johnson A.B.
        • Hirschberg E.
        • Banks J.
        Studies of the Mechanism of Aging of Human Red Blood Cells.
        Ann. New York Acad. Sc. 1958; 75: 95
        • Edwards M.J.
        • Koler R.D.
        • Rigas D.A.
        • Pitcairn D.M.
        The Effect of In Vivo Aging of Normal Human Erythrocytes and Erythrocyte Macromolecules Upon Oxyhemoglobin Dissociation.
        J. Clin. Invest. 1961; 40: 636
        • Furedi A.A.
        • Ohad I.
        Effects of High-Frequency Electric Fields on the Living Cell. I. Behaviour of Human Erythrocytes in High-Frequency Electric Fields and Its Relation to Their Age.
        Biochem. et biophys. acta. 1964; 79: 1
        • Heller V.G.
        • Paul H.
        Changes in Cell Volume Produced by Varying Concentrations of Different Anticoagulants.
        J. Lab. & Clin. Med. 1934; 19: 777
        • Hahn P.F.
        • Balfour W.M.
        • Ross J.F.
        • Bale W.F.
        • Whipple G.H.
        Red Cell Volume Circulating and Total as Determined by Radioiron.
        Science. 1941; 93: 87
        • Danon D.
        A Rapid Micro-Method for Recording Red Cell Osmotic Fragility by Continuous Decrease of Salt Concentration.
        J. Clin. Pathol. 1963; 16: 377
        • Danon D.
        • Frei E.M.
        • Frei Y.E.
        • Lipkin Y.
        An Instrument for Automatically Recording the Osmotic Fragility Curve of Red Cells and/or Its Derivative.
        Tr. Bio-Med. Electronics. 1963; 10: 24
        • de Vries A.
        • Klibansky C.
        • Djaldeti M.
        • Kirschmann C.
        Erythrocyte Phospholipid in Periodic Disease.
        New Istanbul Contr. Clin. Sc. 1963; 6: 77
        • Westerman M.P.
        • Pierce L.E.
        • Jensen W.N.
        Erythrocyte Lipids: A Comparison of Normal Young and Normal Old Populations.
        J. Lab. & Clin. Med. 1963; 63: 394