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Abstract
Density distribution of cells (DDC) can be determined by the described method, in
which blood cells are separated according to their density using phthalate esters
as separating liquids. Methyl phthalate and di-n-butyl phthalate are mixed in different
proportions to yield a battery of fluids with increments of specific gravity of 0.004.
The blood is introduced over the different separating liquids in twenty microhematocrit
capillaries. After centrifugation, denser cells are separated from less dense cells
by a transparent layer of the non-water miscible phthalate esters. The DDC curve thus
obtained represents age population distribution as determined by tagging young red
cells in vivo with Fe59 and following their maturation. The similarity of the DDC curve to the osmotic fragility
curve is shown for normal blood. Various deviations between these two curves occur
in certain pathological conditions. The potential diagnostic value of this method
is suggested.
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References
- Clinical Hematology.ed. 5. Lea & Febiger, 1961
- Differential Density Separation of Cellular Suspension.Anal. Biochem. 1960; 1: 263
- Age as Affecting the Osmotic and Mechanical Fragility of Dog Erythrocytes Tagged With Radioactive Iron.J. Exper. Med. 1950; 91: 147
- Enzyme Activity as a Function of Age in the Human Erythrocyte.Brit. J. Haematol. 1955; 1: 291
- Differences Between Young and Mature Rabbit Erythrocytes.J. Cell. Comp. Physiol. 1956; 47: 215
- The Distribution of Fe59-Tagged Human Erythrocytes in Centrifuged Specimens as a Function of Cell Age.J. Clin. Invest. 1957; 36: 676
- The Aging of Red Cells.J. Physiol. 1958; 143: 325
- On the Relationship of Certain Erythrocyte Characteristics to Their Physiological Age.J. Cell Comp. Physiol. 1958; 51: 415
- Studies of the Mechanism of Aging of Human Red Blood Cells.Ann. New York Acad. Sc. 1958; 75: 95
- The Effect of In Vivo Aging of Normal Human Erythrocytes and Erythrocyte Macromolecules Upon Oxyhemoglobin Dissociation.J. Clin. Invest. 1961; 40: 636
- Effects of High-Frequency Electric Fields on the Living Cell. I. Behaviour of Human Erythrocytes in High-Frequency Electric Fields and Its Relation to Their Age.Biochem. et biophys. acta. 1964; 79: 1
- Changes in Cell Volume Produced by Varying Concentrations of Different Anticoagulants.J. Lab. & Clin. Med. 1934; 19: 777
- Red Cell Volume Circulating and Total as Determined by Radioiron.Science. 1941; 93: 87
- A Rapid Micro-Method for Recording Red Cell Osmotic Fragility by Continuous Decrease of Salt Concentration.J. Clin. Pathol. 1963; 16: 377
- An Instrument for Automatically Recording the Osmotic Fragility Curve of Red Cells and/or Its Derivative.Tr. Bio-Med. Electronics. 1963; 10: 24
- Erythrocyte Phospholipid in Periodic Disease.New Istanbul Contr. Clin. Sc. 1963; 6: 77
- Erythrocyte Lipids: A Comparison of Normal Young and Normal Old Populations.J. Lab. & Clin. Med. 1963; 63: 394
Article info
Publication history
Accepted:
August 10,
1964
Received:
June 10,
1964
Identification
Copyright
© 1964 Published by Elsevier Inc.
