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Abstract
Levels of gamma-2, beta-2A, and beta-2M globulins were measured in human serum by
a modification of Oudin's method of single precipitation in agar. Commercial antisera
to each of the three immunoglobulins formed a single precipitin band with human serum.
This band is specific for the corresponding globulin. The method is sufficiently simple
and reproducible for routine laboratory use. Mean immunoglobulin levels in fifty-four
normal sera were 1,194 mg. per cent for gamma-2 globulin, 395 mg. per cent for beta-2A
globulin, and 191 mg. per cent for beta-2M globulin. Sera from patients with a variety
of clinical conditions, including hypogammaglobulinemia, macroglobulinemia, myeloma,
and lymphoma, contained abnormal amounts of immunoglobulin.
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Article info
Publication history
Accepted:
June 29,
1964
Received:
April 17,
1964
Footnotes
☆Supported in part by United States Public Health Service Research Grants Nos. AM-04532, AM-07529, and AI-03047.
Identification
Copyright
© 1964 Published by Elsevier Inc.
