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Studies on the pathogenesis of thrombosis: An experimental “hypercoagulable” state induced by the intravenous injection of ellagic acid

  • Robert E. Botti
    Footnotes
    Affiliations
    From the Department of Medicine, Western Reserve University School of Medicine, Cleveland, Ohio U.S.A.

    From the University Hospitals of Cleveland Cleveland, Ohio U.S.A.
    Search for articles by this author
  • Oscar D. Ratnoff
    Footnotes
    Affiliations
    From the Department of Medicine, Western Reserve University School of Medicine, Cleveland, Ohio U.S.A.

    From the University Hospitals of Cleveland Cleveland, Ohio U.S.A.
    Search for articles by this author
  • Author Footnotes
    ∗ American Heart Association Research Fellow in Medicine.
    ∗∗ Career Investigator of the American Heart Association.
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      Abstract

      The intravenous injection in dogs of ellagic acid, a commercially available compound, resulted in a prolonged hypercoagulable state. Following the injection, a striking decrease occurred in the clotting time of venous blood as measured in glass or silicone-coated tubes. Consistent with the observation that ellagic acid activates Hageman factor, there was an increase in prothrombin “consumption” during clotting and an acceleration of “thromboplastin generation” in plasma. Thrombosis occurred only when complete stasis of the circulation was deliberately induced. It is hoped that this experimental model will be used to study the influence of other factors upon the induction of thrombosis.
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