This paper is only available as a PDF. To read, Please Download here.
Mice were protected from radiation-induced bone marrow death by shielding a portion of their bone marrow (femoral marrow) from supralethal doses of x-rays. Hemopoietic colonies developed to macroscopic size in the spleen about 4 days after irradiation. Subsequently, atrophic spleens gained mass and exceeded normal spleens in weight during the second week postirradiation. Splenic uptake of 131I-labeled iododeoxyuridine increased during the first 4 days and reached a peak of about twice normal 8 days after irradiation. In contrast, splenic uptake of radioiron (59Fe) did not increase until the fifth day after irradiation. Erythropoietin failed to increase splenic uptake of 59Fe on the fifth day. Peripheral blood cell counts began to increase toward the end of the first week and the beginning of the second week after irradiation. The results were interpreted as evidence that stem cells in a state of cell cycle are not available for erythroid differentiation.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Translational Research
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- Mechanisms of anaemia in leukaemia.Brit. J. Haemat. 1958; 4: 281
- Studies on erythropoiesis. VI. Erythropoietin in human plasma.J. Lab. & Clin. Med. 1957; 50: 534
- Studies of plasma erythropoietic factor in anemic human patients.Blood. 1957; 12: 993
- Control of erythropoiesis.in: Advances in internal medicine. ed. 10. Year Book Medical Publishers, Inc, Chicago1960
- Kinetic model of a bone-marrow stem-cell population.Brit. J. Haemat. 1962; 8: 442
- Recent studies in erythroid differentiation and proliferation.Medicine. 1964; 43: 625
- A method for investigation of stem-cell kinetics.Brit. J. Haemat. 1962; 8: 461
- A direct measurement of the radiation sensitivity of normal mouse bone marrow cells.Radiat. Res. 1961; 14: 213
- An approach to the characterization of stem cells in mouse bone marrow.Ann. New York Acad. Sc. 1964; 114: 571
- Preparation of very high specific activity iododeoxyuridine, iodouridine and iodouracil, labelled with iodine-125, -131 or -132.Nature. 1963; 200: 65
- Marrow transplantation measured by uptake of Fe59 by spleen.Am. J. Physiol. 1964; 206: 1244
- A comparison of erythropoietin bioassays.in: ed. 5. Proc. Soc. Exper. Biol. & Med. 110. 1962: 48
- The mouse with hypoxia-induced erythemia, an erythropoietin hioassay animal.J. Lab. & Clin. Med. 1962; 60: 846
- Deoxyribonucleic acid metabolism in vivo. I. Cell proliferation and death as measured by incorporation and elimination of iododeoxyuridine.in: ed. 5. Fed. Proc. 23. 1964: 640
- Reversion by erythropoietin of the suppression of erythroid clones caused by transfusion induced polycythemia.Transplantation. 1965; 3: 706
- The role of the spleen in radiation injury.in: ed. 5. Proc. Soc. Exper. Biol. & Med. 70. 1949: 740
- Repopulating potential of blood and marrow.J. Clin. Invest. 1964; 43: 1306
- Erythrocyte repopulation in x-irradiated recipients of nucleated, peripheral blood cells of normal mice.in: ed. 5. Proc. Soc. Exper. Biol. & Med. 104. 1960: 722
- Evidence for stem cells in the peripheral blood of mice.Blood. 1962; 19: 702
- Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells.Nature. 1963; 197: 452
- Proliferation of hemopoietic colony-forming cells transplanted into irradiated mice.Radiat. Res. 1964; 22: 383
- Repair processes in irradiated mouse hematopoietic tissue.Ann. New York Acad. Sc. 1964; 114: 115
- Deficient growth of C57BL marrow cells transplanted in F1 hybrid mice.Immunology. 1964; 7: 291
- The effect of differing demands for blood cell production on DNA synthesis by hemopoietic colony-forming cells of mice.Blood. 1966; 26: 296
- Stem-cell kinetics after irradiation.Internat. J. Rad. Biol. 1963; 7: 87
Hodgson, G., and Eskuche, I: Erythropoiesis, a model for the study of factors affecting cell proliferation in the mammal, Nat. Cancer Inst. Monograph No. 14.
- Erythropoietin: Hypothesis of action tested by analog computer.Science. 1966; 152: 367
Accepted: February 16, 1967
Received: January 4, 1967
© 1967 Published by Elsevier Inc.