Research Article| Volume 70, ISSUE 1, P23-35, July 1967

Erythroid differentiation during stem cell proliferation

  • Richard L. DeGowin
    From the Department of Medicine, The University of Chicago Chicago, Ill. U.S.A.

    From the Argonne Cancer Research Hospital (operated by The University of Chicago for The United States Atomic Energy Commission) Chicago, Ill. U.S.A.
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      Mice were protected from radiation-induced bone marrow death by shielding a portion of their bone marrow (femoral marrow) from supralethal doses of x-rays. Hemopoietic colonies developed to macroscopic size in the spleen about 4 days after irradiation. Subsequently, atrophic spleens gained mass and exceeded normal spleens in weight during the second week postirradiation. Splenic uptake of 131I-labeled iododeoxyuridine increased during the first 4 days and reached a peak of about twice normal 8 days after irradiation. In contrast, splenic uptake of radioiron (59Fe) did not increase until the fifth day after irradiation. Erythropoietin failed to increase splenic uptake of 59Fe on the fifth day. Peripheral blood cell counts began to increase toward the end of the first week and the beginning of the second week after irradiation. The results were interpreted as evidence that stem cells in a state of cell cycle are not available for erythroid differentiation.
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