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Abstract
The preparation of polycythemic mice by the use of silicone rubber membranes for erythropoietin
bioassays is reported. The results of hematocrit values, reticulocyte counts, red
blood cell 59Fe incorporations, and responses to erythropoietin illustrate that these animals are
quite similar in their erythropoietin responses to animals made polycythemic by other
techniques. The results presented in this report indicate that for optimum erythropoietin
bioassays, mice should be housed, 11 mice per cage, for 2 to 3 weeks in membrane cages
and assayed 6 to 12 days after their removal. Of the mouse strains investigated, the
C3H/HeJ mouse appears to be the most sensitive to erythropoietin after removal from
the membrane cages.
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References
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Article info
Publication history
Accepted:
February 23,
1967
Received:
January 9,
1967
With the technical assistance of SHEILA LAIL and GAIL COOPERFootnotes
☆Aided in part by National Science Foundation Grant GB 4818 and United States Public Health Service Grant HE 10567-01.
Identification
Copyright
© 1967 Published by Elsevier Inc.
