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Abstract
The absorption of radio-iodinated triolein was compared to that of glyceryl-trioleate-14C in rats. The results obtained showed that there was no difference in the absorption
of these two radioactive fats as determined by the radioactivity in the stool. The
absorption of radio-iodinated triolein mixed with olive oil was 91.7 ± 7.6 per cent
(mean ± S.D.) and of glyceryl-trioleate-14C was 91.6 ± 9.8 per cent. In the stomach, small intestine, cecum and colon, and in
the stool, no deiodination of the radio-iodinated triolein was found. In the small
intestinal wall and in the thoracic duct's lymph, however, massive deiodination of
the 131I labeled fat was probably present. These results suggest that deiodination of the
radio-iodinated triolein occurs after it is absorbed in the gastrointestinal tract.
For this reason it is concluded that radio-iodinated triolein can be used to substitute
glyceryl-trioleate-14C and natural triolein as a test fat in fat absorption studies in the rat if fecal
radioactivity is taken as the index for fat absorption.
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References
- The absorption and disposition of orally administered 131I labeled neutral fat in man.J. Lab. & Clin. Med. 1949; 34: 1634
- The use of 131I triolein in the study of absorption disorders in man.Gastroenterology. 1957; 32: 1
- The use of 131I labeled fat in the study of fat digestion and absorption in normal individuals and in patients with diseases of fat absorption.Gastroenterology. 1957; 32: 17
- Clinical assessment of intestinal fat absorption using radioactive fat.Brit. M. J. 1960; 1: 1403
- Limitations of the 131I labeled triolein tests in the diagnosis of steatorrhea.Gastroenterology. 1962; 42: 16
- An evaluation of the radioactive fecal fat analysis.Am. J. Roentgenol. 1963; 89: 797
- Assessment of the radio-triolein test in steatorrhea.Brit. M. J. 1961; 2: 933
- Importance of the dosing procedure in the radio-triolein test for steatorrhea.Gastroenterology. 1963; 44: 275
- A comparison of laboratory tests in the malabsorption syndrome.Canad. M. A. J. 1964; 90: 116
- Résultats comparés des tests de l'absorption intestinale utilisant la trioléine ou l'acide oléique marqués à l'I131 et au C14.Radio. Isotope Klin. und Forsch. 1964; 6: 337
- Re-evaluation of the 131I-triolein absorption test. Analysis and purification of commercial radio-iodinated triolein and clinical studies with pure preparations.J. Lab. & Clin. Med. 1963; 61: 620
- The impurity of radio-iodinated triolein.J. Clin. Path. 1964; 17: 160
- A simple method for the isolation and purification of total lipids from animal tissues.J. Biol. Chem. 1957; 226: 497
- Removal of fecal pigments and its application to the determination of fecal bile acids in the rat.Anal. Biochem. 1963; 6: 520
- Rapid method for the determination of fat in the feces.J. Biol. Chem. 1949; 177: 347
- Separated channels improve liquid scintillation counting.Nucleonics. 1957; 15: 90
- The fatty acid composition of thoracic lymph fat of rats fed single triglycerides.in: Frazer A.C. International Conference on biochemical problems of lipids. Elsevier Publishing Company, Amsterdam1963: 272-279
Rümke, Chr. L., and Van Eeden, C.: Statistiek voor Medici, Leiden, Uitg. L. Stafleu en Zoon.
- Limitation of radio-iodine as a label for fat.J. Lab. & Clin. Med. 1958; 52: 831
- The stability of radio-iodinated olive oil.Clin. Sc. 1960; 19: 321
- Déshalogénation des graisses halogénées au cours de l'absorption intestinale. Etude expérimentale chez le chien avec la trioléine I131 et l'acide oléique I131.Compt. rend. Soc. biol. 1961; 155: 1705
- The gastric secretion of iodides, a factor limiting the use of 131I-labeled fat in experimental studies on intestinal fat absorption.Acta biochim. Pol. 1963; 10: 337
- The stability of radio-triolein bond in intestinal secretions.Gut. 1961; 2: 131
Article info
Publication history
Accepted:
March 13,
1967
Received:
August 8,
1966
Footnotes
☆This article is based on the first author's academic thesis, University of Groningen: Lipid-soluble inert indicator and radioactive fat in fat absorption studies, 1966.
Identification
Copyright
© 1967 Published by Elsevier Inc.