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The absorption of radio-iodinated triolein was compared to that of glyceryl-trioleate-14C in rats. The results obtained showed that there was no difference in the absorption of these two radioactive fats as determined by the radioactivity in the stool. The absorption of radio-iodinated triolein mixed with olive oil was 91.7 ± 7.6 per cent (mean ± S.D.) and of glyceryl-trioleate-14C was 91.6 ± 9.8 per cent. In the stomach, small intestine, cecum and colon, and in the stool, no deiodination of the radio-iodinated triolein was found. In the small intestinal wall and in the thoracic duct's lymph, however, massive deiodination of the 131I labeled fat was probably present. These results suggest that deiodination of the radio-iodinated triolein occurs after it is absorbed in the gastrointestinal tract. For this reason it is concluded that radio-iodinated triolein can be used to substitute glyceryl-trioleate-14C and natural triolein as a test fat in fat absorption studies in the rat if fecal radioactivity is taken as the index for fat absorption.
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Accepted: March 13, 1967
Received: August 8, 1966
☆This article is based on the first author's academic thesis, University of Groningen: Lipid-soluble inert indicator and radioactive fat in fat absorption studies, 1966.
© 1967 Published by Elsevier Inc.