Advertisement
Research Article| Volume 70, ISSUE 1, P150-157, July 1967

Effects of in vivo hyperoxia on erythrocytes. VI. Hemolysis occurring after exposure to oxygen under high pressure

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Previous studies in this laboratory have shown that tocopherol deficient mice exposed to hyperbaric oxygen developed hemolytic anemia in association with in vivo peroxidation of red cell lipid. The present investigation has shown that lipid peroxides (measured indirectly as malonylaldehyde) form in the red cells of tocopherol deficient mice exposed to hyperbaric oxygen for brief periods of time, during which time no hemolysis occurs. After exposure, while the mice remain at normal atmospheric conditions, hemolysis begins and progresses. With the onset of hemolysis, lipid peroxides appear in, plasma, and the red cell lipid peroxide content decreases indicating that lipid peroxides are released into plasma from damaged red blood cells. These findings, which illustrate that the peroxidation of the red cell lipid occurs before rather than after the onset of hemolysis, support the concept that peroxidation of lipid is responsible for lysis. In addition the results of this study broaden current concepts of oxygen toxicity by demonstrating directly that a manifestation of oxygen toxicity may first appear after there has been a return to normal atmospheric conditions.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Bernheim F.
        • Ottolenghi A.
        • Wilbur K.M.
        Studies on bone marrow lipid in normal and irradiated rabbits.
        Radiat. Res. 1956; 4: 132
        • Ottolenghi A.
        Interaction of ascorbic acid and mitochondrial lipides.
        Arch. Biochem. 1959; 79: 355
        • Wilbur K.M.
        • Bernheim F.
        • Shapiro O.W.
        The thiobarbituric acid reagent as a test for the oxidation of unsaturated fatty acids by various agents.
        Arch. Biochem. 1949; 24: 305
        • Sinnhuber R.O.
        • Yu T.C.
        • Yu T.C.
        Characterization of the red pigment formed in the 2-thiobarbituric acid determination of oxidative rancidity.
        Food Res. 1958; 23: 626
        • Sinnhuber R.O.
        • Yu T.C.
        2-Thiobarbituric acid method for the measurement of rancidity in fishing products. II. The quantitative determination of malonylaldehyde.
        Food Technol. 1958; 12: 9
        • Tsen C.C.
        • Collier H.B.
        The protective action of tocopherol against hemolysis of rat erythrocytes by dialuric acid.
        Canad. J. Biochem. 1960; 38: 957
        • Bunyan J.
        • Green J.
        • Edwin E.E.
        • Diplock A.T.
        Studies on vitamin E. V. Lipid peroxidation of dialuric acid-induced haemolysis of vitamin E-deficient erythrocytes.
        Biochem. J. 1960; 77: 47
        • Taylor D.W.
        • Wiseman R.
        Rate of oxygen uptake of erythrocytes of vitamin E-deficient rats.
        Nature. 1962; 196: 1102
        • Zalkin H.
        • Tappel A.L.
        Studies of the mechanism of vitamin E action. IV. Lipide peroxidation in the vitamin D-deficient rabbit.
        Arch. Biochem. 1960; 88: 113
        • Kann Jr., H.E.
        • Mengel C.E.
        • Smith W.
        • Horton B.
        Oxygen toxicity and vitamin E.
        Aerospace Med. 1964; 35: 840
        • Mengel C.E.
        • Kann H.E.
        • Smith W.W.
        • Horton B.D.
        Effects of in vivo hyperoxia on erythrocytes. I. Hemolysis in mice exposed to hyperbaric oxygenation.
        in: ed. 2. Proc. Soc. Exper. Biol. & Med. 116. 1964: 259
        • Mengel C.E.
        • Kann H.E.
        • Horton B.D.
        Studies of the hemolytic effect of in vivo hyperoxia.
        Clin. Res. 1964; 12: 60
        • Mengel C.E.
        • Kann Jr., H.E.
        Effects of in vivo hyperoxia on erythrocytes. III. In vivo peroxidation of erythrocyte lipid.
        J. Clin. Invest. 1966; 45: 1150
        • Cartwright G.E.
        Diagnostic laboratory hematology.
        ed. 2. Grune & Stratton, Inc, New York1958
        • Townes P.L.
        • Lovell G.R.
        Hereditary methemoglobinemia: A new variant exhibiting dominant inheritance of methemoglobin A.
        Blood. 1961; 18: 18
        • Danon D.
        A rapid micromethod for recording red cell osmotic fragility by continuous decrease of salt concentration.
        J. Clin. Path. 1963; 16: 377
        • Donnan S.K.
        The thiobarbituric acid test applied to tissues from rats treated in various ways.
        J. Biol. Chem. 1950; 182: 415
        • O'Malley B.W.
        • Mengel C.E.
        • Meriwether W.D.
        • Zirkle L.G.
        Inhibition of erythrocyte acetylcholinesterase by peroxides.
        Biochemistry. 1966; 5: 40
        • Gordon H.H.
        • Nitowsky H.M.
        • Cornblath M.
        Studies of tocopherol deficiency in infants and children. I. Hemolysis of erythrocytes in hydrogen peroxide.
        Am. J. Dis. Child. 1955; 90: 669
        • Darby W.J.
        • Cherrington M.E.
        • Ruffin J.M.
        Plasma tocopherol levels in sprue.
        in: ed. 5. Proc. Soc. Exper. Biol. & Med. 63. 1946: 310
        • Silber R.
        • Kayden H.J.
        Vitamin E deficiency and autohemolysis in acanthocytosis.
        Blood. 1965; 26: 895
        • Binder H.J.
        • Herting D.C.
        • Hurst V.
        • Finch S.C.
        • Spiro H.M.
        Tocopherol deficiency in man.
        New England J. Med. 1965; 273: 1289
        • Mengel C.E.
        • Kann Jr., H.E.
        • Heyman A.
        • Metz E.
        Effects of in vivo hyperoxia on erythrocytes. II. Hemolysis in a human after exposure to oxygen under high pressure.
        Blood. 1965; 25: 822
        • Fuson R.L.
        • Saltzman H.A.
        • Smith W.W.
        • Whalen R.E.
        • Osterhout S.
        • Parker R.T.
        Clinical hyperbaric oxygenation with severe oxygen toxicity.
        New England J. Med. 1965; 273: 415
        • Helvey W.M.
        A problem of man and milieu: Prolonged exposure to pure oxygen.
        in: ed. 5. Fed. Proc. 22. 1963: 1057