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Abstract
Previous studies in this laboratory have shown that tocopherol deficient mice exposed
to hyperbaric oxygen developed hemolytic anemia in association with in vivo peroxidation
of red cell lipid. The present investigation has shown that lipid peroxides (measured
indirectly as malonylaldehyde) form in the red cells of tocopherol deficient mice
exposed to hyperbaric oxygen for brief periods of time, during which time no hemolysis
occurs. After exposure, while the mice remain at normal atmospheric conditions, hemolysis
begins and progresses. With the onset of hemolysis, lipid peroxides appear in, plasma,
and the red cell lipid peroxide content decreases indicating that lipid peroxides
are released into plasma from damaged red blood cells. These findings, which illustrate
that the peroxidation of the red cell lipid occurs before rather than after the onset
of hemolysis, support the concept that peroxidation of lipid is responsible for lysis.
In addition the results of this study broaden current concepts of oxygen toxicity
by demonstrating directly that a manifestation of oxygen toxicity may first appear
after there has been a return to normal atmospheric conditions.
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Article info
Publication history
Accepted:
March 21,
1967
Received:
June 17,
1966
Footnotes
☆This work was supported by United States Public Health Service Research Grants CA-08699 and CA-08702 by United States Navy Research Contract Nonr-495 (30) and United States Public Health Service Training Grant 1-T01 CA-5192-01.
☆☆Presented in part at the Midwest Meeting of the American Federation of Clinical Research Chicago, Ill., 1965.
Identification
Copyright
© 1967 Published by Elsevier Inc.