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Previous studies in this laboratory have shown that tocopherol deficient mice exposed to hyperbaric oxygen developed hemolytic anemia in association with in vivo peroxidation of red cell lipid. The present investigation has shown that lipid peroxides (measured indirectly as malonylaldehyde) form in the red cells of tocopherol deficient mice exposed to hyperbaric oxygen for brief periods of time, during which time no hemolysis occurs. After exposure, while the mice remain at normal atmospheric conditions, hemolysis begins and progresses. With the onset of hemolysis, lipid peroxides appear in, plasma, and the red cell lipid peroxide content decreases indicating that lipid peroxides are released into plasma from damaged red blood cells. These findings, which illustrate that the peroxidation of the red cell lipid occurs before rather than after the onset of hemolysis, support the concept that peroxidation of lipid is responsible for lysis. In addition the results of this study broaden current concepts of oxygen toxicity by demonstrating directly that a manifestation of oxygen toxicity may first appear after there has been a return to normal atmospheric conditions.
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Accepted: March 21, 1967
Received: June 17, 1966
☆This work was supported by United States Public Health Service Research Grants CA-08699 and CA-08702 by United States Navy Research Contract Nonr-495 (30) and United States Public Health Service Training Grant 1-T01 CA-5192-01.
☆☆Presented in part at the Midwest Meeting of the American Federation of Clinical Research Chicago, Ill., 1965.
© 1967 Published by Elsevier Inc.