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Studies on the preservation of human blood. II. The relationship of erythrocyte adenosine triphosphate levels and other in vitro measures to red cell storageability

  • Raymond J. Dern
    Affiliations
    From the Department of Medicine, Stritch School of Medicine Chicago, Ill. USA

    From the Department of Hematology of the Hektoen Institute for Medical Research of the Cook County Hospital Chicago, Ill. USA

    From the Department of Medicine (Simpson Memorial Institute) and Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Mich. USA
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  • George J. Brewer
    Affiliations
    From the Department of Medicine, Stritch School of Medicine Chicago, Ill. USA

    From the Department of Hematology of the Hektoen Institute for Medical Research of the Cook County Hospital Chicago, Ill. USA

    From the Department of Medicine (Simpson Memorial Institute) and Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Mich. USA
    Search for articles by this author
  • John J. Wiorkowski
    Affiliations
    From the Department of Medicine, Stritch School of Medicine Chicago, Ill. USA

    From the Department of Hematology of the Hektoen Institute for Medical Research of the Cook County Hospital Chicago, Ill. USA

    From the Department of Medicine (Simpson Memorial Institute) and Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Mich. USA
    Search for articles by this author
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      Abstract

      In a study involving 37 subjects, whole blood was stored at 4 °C. for 21 and 28 days in either ACD or CPD anticoagulant, following which, the in vivo viability of the stored red cells was measured. There was a high degree of correlation (R = 0.95) between the postinfusion viability of stored cells and poststorage adenosine triphosphate (ATP) levels. This indicates the potential use of ATP determinations to predict in vivo red cell viability after in vitro storage. In contract, the correlation between prestorage ATP levels and poststorage R.B.C. viability was not great enough to be of predictive value. Of ten other biochemical and hematological measures, only poststorage osmotic fragility was moderately correlated with poststorage red cell viability.
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