Advertisement
Research Article| Volume 69, ISSUE 6, P979-988, June 1967

Antibodies to cytoplasmic antigens in primary biliary cirrhosis and chronic active hepatitis

      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      Antibodies to cytoplasmic antigens were observed with the fluorescent antibody technique in 79 per cent of 53 sera from patients with primary biliary cirrhosis, in 12 of 15 sera from patients with chronic active hepatitis, in 3 sera from patients with drug hepatitis, in 1 patient with cholestatic viral hepatitis, and in 1 patient with systemic lupus erythematosus. The cytoplasmic antibodies lack organ or species specificity and are best detected with unfixed sections of stomach, submaxillary gland of the rhesus monkey, and hyperactive thyroid. These antibodies are complement fixing, variously distributed in all immunoglobulin fractions and are adsorbed by the more particulated subcellular fractions of liver. They do not correlate with the antibodies detected serologically by the complement fixation text, which are directed against all subcellular fractions of liver. These antibodies to cytoplasmic antigens seem significant in the diagnosis of primary biliary cirrhosis and chronic active hepatitis, and together with the other immunologic alterations observed in these diseases, point to a complex alteration of the immune system.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Paronetto F.
        • Schaffner F.
        • Popper H.
        Immunocytochemical and serologic observations in primary biliary cirrhosis.
        New England J. Med. 1964; 271: 1123
        • Walker J.G.
        • Doniach D.
        • Roitt I.M.
        • Sherlock S.
        Serological tests in diagnosis of primary biliary cirrhosis.
        Lancet. 1965; 1: 827
        • Rubin E.
        • Schaffner F.
        • Popper H.
        Primary biliary cirrhosis. Chronic nonsuppurative destructive cholangitis.
        Am. J. Path. 1965; 46: 387
        • Paronetto F.
        • Horowitz R.
        • Sicular A.
        • Burrows L.
        • Kark A.E.
        • Popper H.
        Immunologic observations on homografts. I. The canine liver.
        Transplantation. 1965; 3: 303
        • Lachmann P.
        • Müller-Eberhard H.J.
        • Kunkel H.G.
        • Paronetto F.
        The localization of in vivo bound complement in tissue sections.
        J. Exper. Med. 1962; 115: 63
        • Paronetto F.
        • Koffler D.
        Immunofluorescent localization of immunoglobulins, complement, and fibrinogen in human diseases. I. Systemic lupus erythematosus.
        J. Clin. Invest. 1965; 44: 1657
        • Pinckard R.N.
        • Weir D.M.
        Antibodies against the mitochondrial fraction of liver after toxic liver damage in rats.
        Clin. & Exper. Immunol. 1966; 1: 33
        • Duve C.de
        • Pressman B.C.
        • Gianetto R.
        • Wattiaux R.
        • Applemans F.
        Tissue fractionation studies.
        Biochem. J. 1955; 60: 604
        • Duve C.de
        • Berthet J.
        Reproducibility of differential centrifugation experiments in tissue fractionation.
        Nature. 1953; 172: 1142
        • Sever J.L.
        Application of a microtechnique to viral serological investigations.
        J. Immunol. 1962; 88: 320
        • Donnelley M.
        Studies in experimental immunology of influenza. VII. An improved complement-fixation technique.
        Australian J. Exper. Biol. M. Sc. 1951; 29: 137
        • Friedman H.S.
        Simplification of certain aspects in the performance and interpretation of complement-fixation tests.
        Am. J. Clin. Path. 1960; 33: 264
        • Deicher H.R.G.
        • Holman H.R.
        • Kunkel H.G.
        Anticytoplasmic factors in sera patients with systemic lupus erythematosus and certain other diseases.
        Arth. & Rheum. 1960; 3: 1
        • Doniach D.
        • Roitt I.M.
        • Walker J.G.
        • Sherlock S.
        Tissue antibodies in primary biliary cirrhosis, active chronic (lupoid) hepatitis, cryptogenic cirrhosis and other liver diseases and their clinical implications.
        Clin. & Exper. Immunol. 1966; 1: 237
        • Barnett E.V.
        • North Jr., A.F.
        • Condemi J.J.
        • Jacox R.F.
        • Vaughan J.H.
        Antinuclear factors in systemic lupus erythematosis and rheumatoid arthritis.
        Ann. Int. Med. 1965; 63: 100
        • Wiedermann G.
        • Doerner M.
        • Miescher P.
        Autoimmunitäte Vorgänge gegen Lebergewebe.
        Schweiz. med. Wchnschr. 1964; 94: 257
        • Gajdusek D.C.
        An “autoimmune” reaction against human tissue antigens in certain acute and chronic diseases. I. Serological investigations.
        Arch. Int. Med. 1958; 101: 9
        • Popper H.
        • Paronetto F.
        • Schaffner F.
        Immune processes in the pathogenesis of liver diseases.
        Ann. New York Acad. Sc. 1965; 124: 781
        • Mackay I.R.
        • Weiden S.
        • Hasker J.
        Autoimmune hepatitis.
        Ann. New York Acad. Sc. 1965; 124: 767
        • Mackay I.R.
        • Taft L.I.
        • Cowling D.C.
        Lupoid hepatitis.
        Lancet. 1956; 2: 1323
      1. Page, A. R., and Good, R. A.: Plasma-cell hepatitis, with special attention to steroid therapy, Am. J. Dis. Child. 99: 288, 1060.

        • Read A.E.
        • Sherlock S.
        • Harrison C.V.
        Active “juvenile” cirrhosis considered as part of a systemic disease and the effect of corticosteroid therapy.
        Gut. 1963; 4: 378
        • Bearn A.G.
        • Kunkel H.G.
        • Slater R.J.
        The problem of chronic liver disease in young women.
        Am. J. Med. 1956; 21: 3
        • Klatskin G.
        Subacute hepatic necrosis and postnecrotic cirrhosis due to anicteric infections with the hepatitis virus.
        Am. J. Med. 1958; 25: 333
        • Dible J.H.
        Degeneration, necrosis and fibrosis in the liver.
        Brit. M. J. 1951; 1: 833
        • Faber V.
        Leukocytic specific antinuclear factors.
        Acta med. scandinav. 1966; 178: 257
        • Johnson S.D.
        • Holborow E.J.
        • Glynn L.E.
        Antibody to smooth muscle in patients with liver disease.
        Lancet. 1965; 2: 878
        • Whittingham S.
        • Irwin J.
        • Mackay I.L.
        • Smalley M.
        Smooth muscle antibody in “autoimmune” hepatitis.
        Gastroenterology. 1966; 51: 499
      2. Paronetto, F.: Unpublished observations.