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Metabolic basis of aminonucleoside nephrosis in rats. II. Inhibition of renal gluconeogenesis by aminonucleoside

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      Abstract

      Inhibition of renal gluconeogenesis from pyruvate, α-oxoglutarate (α-OG), and succinate was demonstrable at 24 hours after a single intravenous injection of aminonucleoside before abnormal proteinuria began. Inhibition of renal gluconeogenesis increased as the nephrotic syndrome developed. Among the substrates tested, inhibition of gluconeogenesis from α-OG was much greater than that from pyruvate, succinate, or fumarate. On the other hand, glucose formation from glycerol was increased in the nephrotic group. Intravenous injection of aminonucleoside decreased the fasting blood glucose level within 4 hours. Decrease of blood glucose level was not due to increased utilization of glucose by muscle.
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      References

        • Yoshida T.
        • Yamashita F.
        • Metcoff J.
        Metabolic basis of aminonucleoside nephrosis in the rats. I. Inhibition of α-oxoglutarate oxidation by aminonucleoside.
        J. Lab. & Clin. Med. 1968; 72: 565
        • Krebs H.A.
        Gluconeogenesis.
        in: ed. 2. Proc. Royal Soc., ser. B. 159. 1964: 545
        • Schrago E.
        • Lardy H.A.
        • Nordlie R.C.
        • Foster D.O.
        Metabolic and hormonal control of phosphoenolpyruvate carboxykinase and malic enzyme in rat liver.
        J. Biol. Chem. 1963; 238: 3188
        • Benoy M.P.
        • Elliot K.A.C.
        Metabolism of lactic and pyruvic acids in normal and tumor tissues: Synthesis of carbohydrate.
        Biochem. J. 1937; 31: 1268
        • Lee J.B.
        • Vance V.K.
        • Cahill Jr., G.F.
        Metabolism of C14 labeled substrates by rabbit kidney-cortex and medulla.
        Am. J. Physiol. 1962; 203: 27
        • Krebs H.A.
        • Yoshida T.
        Renal gluconeogenesis. II. The gluconeogenic capacity of the kidney cortex of various species.
        Biochem. J. 1963; 89: 398
        • Deutsch W.
        An improvement of Warburg's method for cutting tissue slices for respiratory experiments.
        J. Physiol. 1936; 87: 56
        • Krebs H.A.
        • Bennett D.A.A.
        • De Gasquet P.
        • Gascoyne T.
        • Yoshida T.
        Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat kidney cortex slices.
        Biochem. J. 1963; 86: 22
        • Lannigan R.
        • Kark R.
        • Pollak V.E.
        The effect of a single intravenous injection of aminonucleoside of puromycin on the rat kidney: A light and electron microscopic study.
        J. Path. Bacteriol. 1962; 83: 357
        • Vernier R.L.
        • Papermaster B.W.
        • Good R.A.
        Aminonucleoside nephrosis. I. Electron microscopic study of renal lesions in rats.
        J Exper. Med. 1959; 109: 115
        • Metcoff J.
        • Kaiser E.
        • Spargo B.
        Correlation of ultrastructure and enzyme activities in kidneys of rats with aminonucleoside nephrosis.
        in: Proc. II Internat. Congr. Nephrol, Prague1963: 511
        • Kmetec E.
        • Heymann W.
        • Cuppage F.
        • Lewy P.
        Respiration of rat kidney-cortex slices in experimental proteinuria and renal disease.
        Lab. Invest. 1964; 13: 272
        • Kinoshita Y.
        • Hirasawa Y.
        • Watabe C.
        • Mikami A.
        • Takashi G.
        • Kido C.
        • Ito G.
        • Miyakawa T.
        • Nakajima S.
        • Osaka S.
        • Kurita Y.
        Metabolic studies on nephrotic syndrome. I. Metabolic studies on aminonucleoside nephrosis in rats.
        Nippon Naika Gakkai Zasshi. 1963; 52: 21
        • Eisenstein A.B.
        Adrenocortical hormones and carbohydrate synthesis in liver.
        in: ed. 2. Advances in enzyme regulation. vol. 3. Pergamon Press, Inc, Oxford1965: 121