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Research Article| Volume 73, ISSUE 1, P6-16, January 1969

Urine beta-glucuronidase in renal injury. I. Enzyme assay conditions and response to mercuric chloride in rats

  • Donald Coonrod
    Affiliations
    From the Samuel J. Sackett Research Laboratories and the Infectious Diseases-Hypersensitivity Section, Department of Medicine, Northwestern University Medical Center Chicago, Ill., USA
    Search for articles by this author
  • Philip Y. Paterson
    Affiliations
    From the Samuel J. Sackett Research Laboratories and the Infectious Diseases-Hypersensitivity Section, Department of Medicine, Northwestern University Medical Center Chicago, Ill., USA
    Search for articles by this author
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      Abstract

      A method of determining rat urine beta-glucuronidase activity is presented. Rat preputial gland beta-glucuronidase appears in the urine in variable and high concentration. Estimation of the enzyme originating from the urinary tract per se requires removal of these glands. The urine enzyme has a pH optimum of 4.8 to 5.4 and a Michaelis constant of 4.6 × 10−4. A noncompetitive, thermostable, dialyzable inhibitor of beta-glucuronidase is present in rat urine in variable but generally low amounts. Normal rat urine beta-glucuronidase activity is comparable to that of human urine. Intramuscular injection of mercuric chloride produces renal injury and transient elevation of urine beta-glucuronidase activity.
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