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Abstract
A method of determining rat urine beta-glucuronidase activity is presented. Rat preputial
gland beta-glucuronidase appears in the urine in variable and high concentration.
Estimation of the enzyme originating from the urinary tract per se requires removal
of these glands. The urine enzyme has a pH optimum of 4.8 to 5.4 and a Michaelis constant
of 4.6 × 10−4. A noncompetitive, thermostable, dialyzable inhibitor of beta-glucuronidase is present
in rat urine in variable but generally low amounts. Normal rat urine beta-glucuronidase
activity is comparable to that of human urine. Intramuscular injection of mercuric
chloride produces renal injury and transient elevation of urine beta-glucuronidase
activity.
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Article info
Publication history
Accepted:
September 13,
1968
Received:
May 22,
1968
Footnotes
☆This work supported in part by Training Grant AM 05069-12 from the National Institute of Arthritis and Metabolic Diseases.
☆☆Work done during the tenure of a United States Public Health Service Traineeship, Training Grant AM 05069-12 from the National Institute of Arthritis and Metabolic Diseases.
Identification
Copyright
© 1969 Published by Elsevier Inc.