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A method of determining rat urine beta-glucuronidase activity is presented. Rat preputial gland beta-glucuronidase appears in the urine in variable and high concentration. Estimation of the enzyme originating from the urinary tract per se requires removal of these glands. The urine enzyme has a pH optimum of 4.8 to 5.4 and a Michaelis constant of 4.6 × 10−4. A noncompetitive, thermostable, dialyzable inhibitor of beta-glucuronidase is present in rat urine in variable but generally low amounts. Normal rat urine beta-glucuronidase activity is comparable to that of human urine. Intramuscular injection of mercuric chloride produces renal injury and transient elevation of urine beta-glucuronidase activity.
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Accepted: September 13, 1968
Received: May 22, 1968
☆This work supported in part by Training Grant AM 05069-12 from the National Institute of Arthritis and Metabolic Diseases.
☆☆Work done during the tenure of a United States Public Health Service Traineeship, Training Grant AM 05069-12 from the National Institute of Arthritis and Metabolic Diseases.
© 1969 Published by Elsevier Inc.