Research Article| Volume 73, ISSUE 1, P42-53, January 1969

Energetics of human blood platelets: Uncoupling of oxidative phosphorylation in intact cells

      This paper is only available as a PDF. To read, Please Download here.


      Platelets stored for short periods lose in vivo viability. Platelet ATP declines concurrently, but it is not known whether its decline is due to accelerated degradation, slowed synthesis, or both. Accordingly, the rate of incorporation of 32Pi into organic compounds (which reflects ATP synthesis) was measured by a modification of the method of Wadkins and Lehninger. Concurrent measurement of ATP levels allowed an estimate of its rate of degradation. Platelets suspended in their own plasma maintained ATP levels for at least 3 hours, during which time the rate of incorporation of 32Pi constantly increased. Thus, ATP levels were maintained at the expense of increasing synthesis. Washed platelets showed a fall to about 50 per cent of initial values in 3 hours. This was accompanied by a decreasing rate of incorporation of 32Pi. This decrease was not solely due to washing, as washed platelets resuspended in plasma behaved similarly to platelets maintained in their own plasma. Glucose, added to washed platelets, stimulated the synthesis of ATP for about one hour, after which it declined. Palmitate, citrate, and succinate, although rapidly oxidized, did not stimulate the synthesis of ATP. This was apparently due to uncoupling of oxidation from phosphorylation. This uncoupling may have resulted from washing and centrifuging the cells, or could have been due to the anticoagulant used (ACD). It was concluded that in washed platelets the predominant source of energy is glycolysis, but that this is probably a consequence of the in vitro uncoupling of oxidative phosphorylation. In addition, there may be plasma factors that stabilize the metabolism of platelets.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Translational Research
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Luganova I.S.
        • Seits I.F.
        • Teodorovich V.I.
        Metabolism in human thrombocytes.
        Biokhimiia. 1958; 23: 379
        • Born G.V.R.
        • Esnouf M.P.
        Biochemical changes in platelet-rich plasma during clotting.
        in: Deutsch E. Proc. Fourth Internat. Cong. Biochem. X. Pergamon Press, Inc, New York1959: 97-104
        • Waller H.D.
        • Löhr G.W.
        • Grignani F.
        • Gross R.
        Über den energiestoffwechsel normaler menschlicher thrombozyten.
        Thromb. et diath. Haemorrh. 1959; 3: 520
        • Bettex-Galland M.
        • Luscher E.F.
        Studies on the metabolism of human blood platelets in relation to clot retraction.
        Thromb. et Diath. Haemorrh. 1960; 4: 178
        • Campbell E.W.
        • Small W.J.
        • LoPilato E.
        • Dameshek W.
        Glycolytic intermediates of human platelets: Their separation and identification.
        in: ed. 2. Proc. Soc. Exper. Med. & Biol. 94. 1957: 505
        • Rossi E.C.
        • Clatanoff D.V.
        Recovery of platelet phosphorus from serum after recalcification of platelet-rich plasma.
        J. Lab. & Clin. Med. 1963; 62: 736
        • Holmsen H.
        Incorporation in vitro of P32 into blood platelet acid-soluble organophosphates and their chromatographic identification.
        Scandinav. J. Clin. & Lab. Invest. 1965; 17: 230
        • Rossi E.C.
        Effects of ethylenediaminetetraacetate (EDTA) and citrate upon platelet glycolysis.
        J. Lab. & Clin. Med. 1967; 69: 204
        • Zucker M.B.
        • Borrelli J.
        Changes in platelet adenosine triphosphate concentration and phosphate distribution during viscous metamorphosis and clot retraction.
        in: Johnson S.A. Monto R.W. Rebuck J.W. Horn Jr., R.C. Henry Ford Hospital International Symposium: Blood platelets. Little, Brown & Company, Boston1961: 383-391
        • Karpatkin S.
        Studies on human platelet glycolysis. Effect of glucose, cyanide, insulin, citrate, and agglutination and contraction on platelet glycolysis.
        J. Clin. Invest. 1967; 46: 409
        • Warshaw A.L.
        • Laster L.
        • Shulman N.R.
        The stimulation by thrombin of glucose oxidation in human platelets.
        J. Clin. Invest. 1966; 45: 1923
        • Bull B.S.
        • Schneiderman M.A.
        • Brecher G.
        Platelet counts with the Coulter counter.
        Am. J. Clin. Path. 1965; 44: 678
        • Galanos D.
        • Kapoulas V.K.
        Rapid method for the determination of organic nitrogen and phosphorus based on a single perchloric acid digestion.
        Anal. chim. acta. 1966; 34: 360
        • Nielsen S.O.
        • Lehninger A.L.
        Phosphorylation coupled to the oxidation of ferro-cytochrome c.
        J. Biol. Chem. 1955; 215: 555
        • Wadkins C.L.
        • Lehninger A.L.
        Preparation and assay of phosphorylating submitochondrial particles.
        in: ed. 2. Methods in enzymology. vol. VI. Academic Press, Inc, New York1963: 268-269
        • Bray G.A.
        A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter.
        Anal. Biochem. 1960; 1: 279
        • Beutler E.
        • Baluda M.C.
        Simplified determination of blood adenosine triphosphate using the firefly system.
        Blood. 1964; 23: 688
        • Melchinger D.
        • Nemerson Y.
        Randomness of platelet adhesiveness with respect to their ATP, O2 consumption, and size.
        J. Appl. Physiol. 1967; 22: 197
        • Bancroft H.
        Introduction to biostatistics.
        Paul B. Hoeber, New York1957
        • Goodman D.S.
        The interaction of human serum albumin with long-chain fatty anions.
        J. Am. Chem. Soc. 1958; 80: 3892
        • Chernyak N.B.
        Processes of formation and utilization of energy in human blood platelets.
        Clin. chim. acta. 1965; 12: 244
        • Zieve P.D.
        • Gamble J.L.
        • Jackson D.P.
        Effects of thrombin on the potassium and ATP content of platelets.
        J. Clin. Invest. 1964; 43: 2063
        • Gross R.
        Metabolic aspects of normal and pathological platelets.
        in: Johnson S.A. Monto R.W. Rebuck J.W. Horn Jr., R.C. Henry Ford Hospital International Symposium: Blood platelets. Little, Brown & Company, Boston1961: 407-422
        • Corn M.
        Effect of thrombin on glycolysis of fresh and stored platelets.
        J. Appl. Physiol. 1966; 21: 62
        • Aster R.H.
        • Jandl J.H.
        Platelet sequestration in man. I. Methods.
        J. Clin. Invest. 1964; 43: 843
        • Rosenzweig A.
        • Ways P.
        The oxidation of long-chain fatty acids by the formed elements of human blood.
        Blood. 1966; 27: 57
        • Donabedian R.K.
        • Nemerson Y
        Fatty acid oxidation by human platelets and its stimulation by thrombin.
        Clin. Res. 1968; 16: 302
        • Laws J.O.
        • Strickland L.H.
        The effect of washing procedures on the phosphate metabolism of Ehrlich ascites-tumor cells.
        Biochem. J. 1963; 87: 520
        • Aldridge W.N.
        • Street B.W.
        Mitochondria from brown adipose tissue.
        Biochem. J. 1968; 107: 315
        • Holmsen H.
        Collagen-induced release of adenosine diphosphate from blood platelets incubated with radioactive phosphate in vitro.
        Scandinav. J. Clin. & Lab. Invest. 1965; 17: 239
        • Holmsen H.
        Changes in the radioactivity of P32-labeled acid-soluble organophosphates in blood platelets during collagen- and adenosine diphosphate-induced platelet aggregation.
        Scandinav. J. Clin. & Lab. Invest. 1965; 17: 537
        • Ireland D.M.
        Effect of thrombin on the radioactive nucleotides of human washed platelets.
        Biochem. J. 1967; 105: 857
        • Schrier S.L.
        Organization of enzymes in human erythrocyte membranes.
        Am. J. Physiol. 1966; 210: 139
        • Cooper C.
        • Lehninger A.L.
        Oxidative phosphorylation by an enzyme complex from extracts of mitochondria. V. The adenosine triphosphate-phosphate exchange reaction.
        J. Biol. Chem. 1957; 224: 561
        • Verdier C.H.D.
        Exchange of phosphate-groups between inorganic phosphate and adenosine triphosphate in red blood cells.
        Acta physiol. scandinav. 1963; 57: 301