Research Article| Volume 73, ISSUE 1, P54-59, January 1969

Disappearance of adenosine diphosphate in vivo

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      ADP (12 mg. per kilogram in 8 ml.) was injected rapidly into the pulmonary artery of live dogs. This produced marked hypotension in the pulmonary artery persisting for 30 minutes and in the systemic circulation for 5 minutes. No increase in plasma ADP, however, could be found 1 to 2 minutes after injection. Decreased heart rate persisted for 10 minutes, but increased ventilation rate and decreased tidal volume were noted for only 1 minute after ADP injection. Additional quantitative studies demonstrated that ADP passed through the pulmonary circulation in large amounts but could not be detected after 1 minute in vivo. ADP levels decrease rapidly in the intact animal compared to ADP levels in vitro.
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        • Angelakos E.T.
        • Glassman P.M.
        Hypotensive and cardiac effects of adenosine and its phosphorylated derivatives.
        Arch. internat. pharmacodyn. 1965; 154: 82
        • Frohlich E.D.
        Local effect of adenosine mono-, di-, and triphosphate vessel resistance.
        Am. J. Physiol. 1963; 204: 28
        • Gordon D.B.
        Basis of the depressor action of adenosine phosphates.
        Am. J. Physiol. 1961; 201: 1126
        • Gordon D.B.
        • Heese D.H.
        Blood pressure lowering action of adenosine diphosphate and related compounds.
        Am. J. Physiol. 1961; 201: 1123
        • Green H.N.
        • Stoner H.B.
        Biological actions of the adenine nucleotides.
        in: H. K. Lewis & Co., Ltd, London1950: 68
        • Adam H.
        Adenosine-5′-diphosphate and adenosine-5′-monophosphate.
        in: Bergmeyer H.U. Methods of enzymatic analysis. Academic Press, Inc, New York1965: 573
        • Ireland D.M.
        • Mills D.C.B.
        Detection and determination of adenosine diphosphate and related substances in plasma.
        Biochem. J. 1966; 99: 283
        • Ireland D.M.
        • Mills D.C.B.
        Degradation of adenosine diphosphate in plasma.
        Biochem. J. 1964; 92: 30P
        • Holmsen H.
        • Stormorken H.
        Kinetic studies on the breakdown of adenosine diphosphate in human plasma.
        Scandinav. J. Clin. & Lab. Invest. (Suppl. 84). 1965; 17: 138
        • Cain D.F.
        • Davies R.E.
        Breakdown of adenosine triphosphate during a single contraction of working muscle.
        Biochem. & Biophys. Res. Comm. 1962; 5: 361
        • Brashear R.E.
        • Ross J.C.
        • Smith C.N.
        Rest and exercise plasma nucleotides.
        Resp. Physiol. 1968; 6 (In press)
        • Winer B.J.
        Statistical principles in experimental design.
        in: McGraw-Hill Book Co, New York1962: 89
        • Dittmer D.S.
        Blood and other body fluids.
        in: Federation of American Societies for Experimental Biology, Washington, D. C1961: 3
        • Born G.V.R.
        • Haslam R.J.
        • Goldman M.
        • Lowe R.D.
        Comparative effectiveness of adenosine analogues as inhibitors of blood-platelet aggregation and as vasodilators in man.
        Nature. 1965; 205: 678
        • Green H.N.
        • Stoner H.B.
        Biological actions of the adenine nucleotides.
        in: H. K. Lewis & Co., Ltd, London1950: 95
        • Reeves J.T.
        • Jokl P.
        • Merida J.
        • Leathers J.E.
        Pulmonary vascular obstruction following administration of high-energy nucleotides.
        J. Appl. Physiol. 1967; 22: 475
        • Brashear R.E.
        • Ross J.C.
        Hemodynamic effects and disappearance of ADP.
        J. Clin. Invest. 1968; 47: 10a
      1. Brashear, R. E., and Ross, J. C.: Hemodynamic effects of ADP. In preparation.

        • Bianchi A.
        • De Natale G.
        • Giaquinto S.
        The effects of adenosine and its phosphorylated derivatives upon the respiratory apparatus.
        Arch. internat. pharmacodyn. 1963; 145: 498
        • Folkow B.
        The vasodilator action of adenosine triphosphate.
        Acta physiol. scandinav. 1949; 17: 311
        • Webster H.L.
        Direct deamination of adenosine diphosphate by washed myofibrils.
        Nature. 1953; 172: 453
        • Sapirstein L.A.
        Fractionation of the cardiac output of rats with isotopic potassium.
        Circulation Res. 1956; 4: 689