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In an effort to develop an immunoassay for erythropoietin (ESF), antisera were produced by a variety of methods. The antibodies active against ESF were found to possess the following characteristics: (1) inability to cause precipitation, (2) ability to fix complement, (3) capability of producing passive cutaneous anaphylaxis, (4) presence in the slowly sedimenting peak on sucrose density ultracentrifugation. By the use of in vivo neutralization tests and hemagglutination studies, two different types of antibodies to erythropoietin were recognized. Type I neutralized the biological activity and type II caused hemagglutination. Some antisera contained both antibody types. Erythropoietin was detected in serum obtained from hematologically normal individuals by a hemagglutination-inhibition technique, and an erythropoietin content from 7 to 30 mU. per milliliter was found. Further study is required to correlate abnormal erythropoietin levels with results of polycythemic mouse assays.
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Accepted: September 25, 1968
Received: July 22, 1968
☆Supported in part by United States Public Health Service Research Grant No. 1 R01 HE-10567-03 from the National Heart Institute, Research Grant No. GB-4818 from the National Science Foundation, and Contract No. AT-(40-1)-3547 from the Atomic Energy Commission.
☆☆Presented in part at the XII Congress International Society Hematology in New York, Sept., 1968.
© 1969 Published by Elsevier Inc.