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Abstract
In an effort to develop an immunoassay for erythropoietin (ESF), antisera were produced
by a variety of methods. The antibodies active against ESF were found to possess the
following characteristics: (1) inability to cause precipitation, (2) ability to fix
complement, (3) capability of producing passive cutaneous anaphylaxis, (4) presence
in the slowly sedimenting peak on sucrose density ultracentrifugation. By the use
of in vivo neutralization tests and hemagglutination studies, two different types
of antibodies to erythropoietin were recognized. Type I neutralized the biological
activity and type II caused hemagglutination. Some antisera contained both antibody
types. Erythropoietin was detected in serum obtained from hematologically normal individuals
by a hemagglutination-inhibition technique, and an erythropoietin content from 7 to
30 mU. per milliliter was found. Further study is required to correlate abnormal erythropoietin
levels with results of polycythemic mouse assays.
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Article info
Publication history
Accepted:
September 25,
1968
Received:
July 22,
1968
Footnotes
☆Supported in part by United States Public Health Service Research Grant No. 1 R01 HE-10567-03 from the National Heart Institute, Research Grant No. GB-4818 from the National Science Foundation, and Contract No. AT-(40-1)-3547 from the Atomic Energy Commission.
☆☆Presented in part at the XII Congress International Society Hematology in New York, Sept., 1968.
Identification
Copyright
© 1969 Published by Elsevier Inc.