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A method for assay of plasma coagulation factor XIII (fibrin stabilizing factor) is described using assay clots incorporating 125I-labeled fibrin. After exposure to solvent, the assay end point is quantified by determinations of supernatant radioactivity. Objective determinations of assay end point yields excellent methodological reproducibility. Normal men and women showed similar plasma factor XIII concentrations, and long-term administration of birth control medication did not influence factor XIII concentrations in the female. In contrast, plasma factor XIII concentration diminished steadily during pregnancy reaching a level approximately 50 per cent of normal at term (p < 0.001). The regression between diminution in factor XIII concentrations and duration of pregnancy was highly significant (r = 0.79, p < 0.001).
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Accepted: October 7, 1968
Received: August 30, 1968
☆This work was supported by Grants HE-03745 and TI-HE-05814 from the National Heart Institute, and Grant TIAM-5312 from the National Institute of Arthritis and Metabolic Disease, Bethesda, Md.
© 1969 Published by Elsevier Inc.