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A simple, isotopic method for the assay of alanine transaminase has been developed involving toluene extraction of the 2,4-dinitrophenylhydrazone of pyruvate-14C formed from alanine-14C. The method has been applied to rat liver, rat serum, human serum, and partially purified enzyme from human liver. A high degree of correlation was observed between the isotopic assay and the method of Reitman and Frankel for the measurement of alanine transaminase activity in human serum.
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Accepted: September 13, 1968
Received: June 14, 1968
☆These studies were supported in part by CCNSC Contract PH-43-66-541 from the National Cancer Institute, National Institutes of Health.
© 1969 Published by Elsevier Inc.