An isotopic assay for alanine transaminase

Allen L. Pollock; Ellen Loehr; Dewayne Roberts

doi:10.5555/uri:pii:0022214369900687

Research Article| Volume 73, ISSUE 1, P166-172, January 1969

An isotopic assay for alanine transaminase

Allen L. Pollock
Allen L. Pollock
Affiliations
From the Laboratory of Pharmacology, The Children's Cancer Research Foundation, Harvard Medical School Boston, Mass., USA
From the Department of Biology, Northeastern University Boston, Mass., USA
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Ellen Loehr
Ellen Loehr
Affiliations
From the Laboratory of Pharmacology, The Children's Cancer Research Foundation, Harvard Medical School Boston, Mass., USA
From the Department of Biology, Northeastern University Boston, Mass., USA
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Dewayne Roberts
Dewayne Roberts
Correspondence
Reprint requests: Dr. DeWayne Roberts, St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, Tenn. 38101.
Affiliations
From the Laboratory of Pharmacology, The Children's Cancer Research Foundation, Harvard Medical School Boston, Mass., USA
From the Department of Biology, Northeastern University Boston, Mass., USA
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DOI:https://doi.org/10.5555/uri:pii:0022214369900687

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Abstract

A simple, isotopic method for the assay of alanine transaminase has been developed involving toluene extraction of the 2,4-dinitrophenylhydrazone of pyruvate-¹⁴C formed from alanine-¹⁴C. The method has been applied to rat liver, rat serum, human serum, and partially purified enzyme from human liver. A high degree of correlation was observed between the isotopic assay and the method of Reitman and Frankel for the measurement of alanine transaminase activity in human serum.

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Article info

Publication history

Accepted: September 13, 1968

Received: June 14, 1968

Footnotes

☆These studies were supported in part by CCNSC Contract PH-43-66-541 from the National Cancer Institute, National Institutes of Health.

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DOI: https://doi.org/10.5555/uri:pii:0022214369900687

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An isotopic assay for alanine transaminase

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