Research Article| Volume 73, ISSUE 1, P173-180, January 1969

Continuous measurement of pentose phosphate pathway activity in erythrocytes. An ionization chamber method

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      A new and simple technique has been devised which allows, for the first time, the instantaneous and continuous measurement of pentose phosphate pathway activity in intact erythrocytes. This technique, employing the vibrating reed electrometer and ionization chamber, measures the oxidation of 14C-labeled substrates to 14CO2. Substrate oxidation curves are described for glucose-1-14C, glucose-2-14C, glucose-3-4-14C, and glucose-6-14C in normal human erythrocytes. Glucose-1-14C oxidation to 14CO2 has been measured in cells from patients with red cell G-6-PD deficiency and other hemolytic anemias. The effects of stimulation with methylene blue on pentose phosphate pathway in normal and diseased red cells are also described.
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