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Abstract
A new and simple technique has been devised which allows, for the first time, the
instantaneous and continuous measurement of pentose phosphate pathway activity in
intact erythrocytes. This technique, employing the vibrating reed electrometer and
ionization chamber, measures the oxidation of 14C-labeled substrates to 14CO2. Substrate oxidation curves are described for glucose-1-14C, glucose-2-14C, glucose-3-4-14C, and glucose-6-14C in normal human erythrocytes. Glucose-1-14C oxidation to 14CO2 has been measured in cells from patients with red cell G-6-PD deficiency and other
hemolytic anemias. The effects of stimulation with methylene blue on pentose phosphate
pathway in normal and diseased red cells are also described.
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References
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Article info
Publication history
Accepted:
September 19,
1968
Received:
July 17,
1968
Footnotes
☆Presented in part at the Western Society for Clinical Research Meeting, Carmel, Calif., Feb. 2, 1968.1
☆☆This investigation was supported by United States Public Health Service Research Grants HE 09472 and HE 08757 from the National Heart Institute.
Identification
Copyright
© 1969 Published by Elsevier Inc.
