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Abstract
Albumin in tissue is particularly susceptible to solubilization in aqueous solutions.
Because of this and the consequent diffusion artifacts, studies of the distribution
of serum albumin in tissue sections have usually been unrewarding. A freeze-substitution
technique was therefore applied to specimens of renal tissue obtained by percutaneous
renal biopsy. With the use of this technique, serum proteins, including albumin, were
fixed effectively in situ without apparent diffusion artifacts. A fluorescein isothiocyanate-conjugated
rabbit antihuman albumin antiserum was used to study the distribution of serum albumin
in 30 renal biopsy specimens from patients with diverse diseases affecting the kidney.
A striking finding was the universal distribution of albumin in the interstitial space,
both within the peritubular capillaries and in the extravascular compartment, thereby
confirming physiologic observations on the extravascular distribution of albumin in
the kidney. In the glomerulus albumin was observed invariably in the glomerular capillaries
and frequently in both mesangium and visceral epithelial cells; it was detected in
Bowman's space in only 8 of 30 specimens. Albumin was detected frequently as discrete
droplets in the lumen and cytoplasm of the proximal convoluted tubules; droplets of
albumin were found only occasionally in cells of the distal convolutions, loops of
Henle, and collecting ducts. A common finding in the distal parts of the nephron was
a diffuse distribution of albumin in the cytoplasm of single or multiple cells or
in the intercellular spaces. These observations suggest that, in addition to proximal
tubular reabsorption, albumin is transported through the cells of distal convolutions,
loops of Henle, and collecting ducts.
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Post, R. S.: Personal communication.
Article info
Publication history
Accepted:
February 11,
1970
Received:
October 7,
1969
Footnotes
☆Supported by Grants AM10314 and AM12330 from the National Institutes of Health and by the Henry P. Shapiro Renal Fund.
Identification
Copyright
© 1970 Published by Elsevier Inc.
