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Abstract
To quantitate deoxyribonucleic acid (DNA) synthesis by circulating human blood mononuclear
leukocytes, buffy coat suspensions were incubated with tritiated thymidine in vitro,
and incorporation was measured by liquid-scintillation counting. Elevated mononuclear
leukocyte DNA synthesis was found in a wide spectrum of inflammatory diseases, including
2 patients with agammaglobulinemia and infection. Increased thymidine uptake was found
especially in the acute phase of disease and tended to decrease with, clinical improvement
of the patient. Uptake was lower in patients with chronic inflammatory diseases. No
correlation was found between the mononuclear leukocyte thymidine uptake and the erythrocyte
sedimentation rate or the total mononuclear cell count. Autoradiographic studies revealed
that the tritium-labeled leukocytes had the appearance of atypical lymphocytes and
did not phagocytize latex particles. Similar labeled mononuclear cells were found
in the blood of a patient with Swiss-type agammaglobulinemia and pneumonia. It was
suggested from the data obtained that proliferating cells may appear in the blood
in response to inflammation. Some may represent lymphoid cells which participate in
an immune response associated with the disease process involved, and some may be precursors
of monocytes released in response to acute tissue injury.
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Article info
Publication history
Accepted:
June 10,
1970
Received:
December 12,
1969
Footnotes
☆Parts of this work have been presented to the American Rheumatism Association1 and published in abstract form.2
☆This work was supported by United States Public Health Service Project Grant AM-09989 and Training Grant AM-05154, and an Arthritis Clinical Research Center Grant from the Arthritis Foundation.
Identification
Copyright
© 1970 Published by Elsevier Inc.