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Phagocytosis of crystalline calcium pyrophosphate dihydrate (CPPD), but not amorphous calcium pyrophosphate, by human polymorphonuclear leukocytes (PMN) resulted in the generation of nondialyzable chemotactic activity. CPPD crystals did not generate as much chemotactic activity from PMN as urate crystals; however, this may have been due to a lower level of PMN phagocytosis of CPPD crystals. Colchicine caused a 70 to 100 per cent reduction in chemotactic activity in urate crystal experiments and 49 to 70 per cent reduction in the remaining 3 experiments. By contrast, in no experiment with CPPD crystals was there greater than 70 per cent inhibition and in 4 experiments there was only 0 to 11 per cent inhibition. Diamond dust crystals were phagocytized by PMN, but no chemotactic activity was found. Release of chemotactic activity, therefore, is not dependent solely on crystal phagocytosis. Diamond dust crystals injected into canine joints did not induce an acute inflammatory response.
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Accepted: July 8, 1970
Received: March 13, 1970
☆Supported in part by Grants AM-12593 and FR-107 from the National Institutes of Health, Bethesda, Maryland, and a grant from the Eastern Pennsylvania Chapter of the Arthritis Foundation.
© 1970 Published by Elsevier Inc.