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Research Article| Volume 76, ISSUE 3, P403-415, September 1970

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Polymorphonuclear leukocyte motility in vitro. V. Release of chemotactic activity following phagocytosis of calcium pyrophosphate crystals, diamond dust, and urate crystals

  • Rose L. Tse
    Affiliations
    From the Rheumatology Research Laboratory, Philadelphia General Hospital, and the Department of Medicine, University of Pennsylvania Philadelphia, Pa. U.S.A.
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  • Paulding Phelps
    Correspondence
    Reprint requests: Dr. Paulding Phelps, General Clinical Research Center, Philadelphia General Hospital, 34th and Civic Center Blvd., Philadelphia, Pa. 19104.
    Affiliations
    From the Rheumatology Research Laboratory, Philadelphia General Hospital, and the Department of Medicine, University of Pennsylvania Philadelphia, Pa. U.S.A.
    Search for articles by this author
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      Abstract

      Phagocytosis of crystalline calcium pyrophosphate dihydrate (CPPD), but not amorphous calcium pyrophosphate, by human polymorphonuclear leukocytes (PMN) resulted in the generation of nondialyzable chemotactic activity. CPPD crystals did not generate as much chemotactic activity from PMN as urate crystals; however, this may have been due to a lower level of PMN phagocytosis of CPPD crystals. Colchicine caused a 70 to 100 per cent reduction in chemotactic activity in Math Eq urate crystal experiments and 49 to 70 per cent reduction in the remaining 3 experiments. By contrast, in no experiment with CPPD crystals was there greater than 70 per cent inhibition and in 4 experiments there was only 0 to 11 per cent inhibition. Diamond dust crystals were phagocytized by PMN, but no chemotactic activity was found. Release of chemotactic activity, therefore, is not dependent solely on crystal phagocytosis. Diamond dust crystals injected into canine joints did not induce an acute inflammatory response.
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