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Research Article| Volume 76, ISSUE 3, P426-431, September 1970

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Tissue localization of the heme-hemopexin complex in the rabbit and the rat as studied by light microscopy with the use of radioisotopes

  • Ursula Muller-Eberhard
    Correspondence
    Reprint requests: Dr. Ursula Muller-Eberhard, Dept. of Biochemistry, Scripps Clinic and Research Foundation, 476 Prospect St., La Jolla, Calif. 92037.
    Footnotes
    Affiliations
    From the Departments of Biochemistry and Experimental Pathology, Scripps Clinic and Research Foundation La Jolla, Calif. U.S.A.
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  • Cesare Bosman
    Affiliations
    From the Departments of Biochemistry and Experimental Pathology, Scripps Clinic and Research Foundation La Jolla, Calif. U.S.A.
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  • Ham Heng Liem
    Affiliations
    From the Departments of Biochemistry and Experimental Pathology, Scripps Clinic and Research Foundation La Jolla, Calif. U.S.A.
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  • Author Footnotes
    ∗ Recipient of Research Career Development Award 2-K3-AM-16, 923 from the National Institute of Arthritis and Metabolic Diseases, United States Public Health Service,
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      Abstract

      The hepatocytes of the rabbit remove plasma heme as the heme-hemopexin complex. This was shown by intravenously injecting tracer amounts of radioactively labeled heme or hemopexin and analyzing tissue slides by radioautography 15, 60, or 120 minutes thereafter. Heme, labeled with 3H or 59Fe, was injected as heme,
      Heme = ferriprotoporphyrin IX.
      Heme = ferriprotoporphyrin IX.
      heme-hemopexin, heme-albumin, or cyanmethemoglobin. Rabbit hemopexin, labeled with 125I, was given as the heme-hemopexin complex. Regardless of which form of heme was administered and whether heme or hemopexin was labeled, all radioactive material was found exclusively in the parenchymal cells of the liver; spleen, kidney, lung, and bone marrow cells remained free of radioactivity.
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