This paper is only available as a PDF. To read, Please Download here.
The hepatocytes of the rabbit remove plasma heme as the heme-hemopexin complex. This was shown by intravenously injecting tracer amounts of radioactively labeled heme or hemopexin and analyzing tissue slides by radioautography 15, 60, or 120 minutes thereafter. Heme, labeled with 3H or 59Fe, was injected as heme,
†Heme = ferriprotoporphyrin IX.heme-hemopexin, heme-albumin, or cyanmethemoglobin. Rabbit hemopexin, labeled with 125I, was given as the heme-hemopexin complex. Regardless of which form of heme was administered and whether heme or hemopexin was labeled, all radioactive material was found exclusively in the parenchymal cells of the liver; spleen, kidney, lung, and bone marrow cells remained free of radioactivity.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Translational Research
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- The binding of haematin by serum proteins.Brit. J. Haemat. 1960; 6: 160
- On plasma proteins with heme or hemoglobin binding capacity.Scand. J. Clin. Lab. Invest. 1960; 12: 121
- Exchange of heme between human hemopexin, human albumin, rabbit hemopexin and rabbit albumin.in: Fed. Proc. 27. 1968: 723 (Abst.)
- Plasma heme-binding in patients with hemolytic disorders.J. Lab. Clin. Med. 1968; 71: 484
- Studies on the disposal of intravascular heme in the rabbit.J. Lab. Clin. Med. 1969; 73: 210
- Disposal of plasma heme in normal man and patients with intravascular hemolysis.J. Clin. Invest. 1970; 49: 5
- Plasma concentrations of hemopexin, haptoglobin and heme in patients with various hemolytic diseases.Blood. 1968; 32: 811
- Depletion of plasma hemopexin in man by hematin injections.in: Proc. Soc. Exp. Biol. Med. 131. 1969: 371
- Studies on the relations between molecular and functional properties of hemoglobin. II. The effect of salts on the oxygen equilibrium of human hemoglobin.J. Biol. Chem. 1961; 236: 397
- Preparation, properties and specific recombination of αβ-globin subunits.J. Biol. Chem. 1964; 239: 3699
- Purification and partial characterization of human hemopexin.J. Lab. Clin. Med. 1967; 70: 619
- A method of trace iodination of proteins for immunologic studies.Int. Arch. Allerg. 1966; 29: 185
- Techniques of autoradiography.Elsevier Publishing Company, Amsterdam1969
- Exchange of heme among hemoglobins and between hemoglobin and albumin.J. Biol. Chem. 1968; 243: 465
- The sites of hemoglobin catabolism.Blood. 1965; 26: 705
- Intracellular catabolism of hemoglobin and iron dextran by the rat liver.J. Lab. Clin. Med. 1969; 73: 181
Accepted: June 17, 1970
Received: March 9, 1970
☆This work was supported by research grants from the National Heart Institute (HE 08660-07), the Institute of Child Health and Human Development (HD 04445-01), the National Institute of Allergy and Infectious Diseases (A1-07007), and the San Diego County Heart Association (No. 83).
© 1970 Published by Elsevier Inc.