Research Article| Volume 76, ISSUE 3, P432-438, September 1970

Muscle copper, zinc, and manganese levels in Wilson's disease: Studies with the use of neutron-activation analysis

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      Fourteen patients with Wilson's disease, 10 of their parents and siblings, and 12 control subjects had muscle copper, zinc, and manganese concentrations determined by the use of neutron-activation analysis. Zinc and manganese concentrations did not differ significantly in the 3 groups. Although patients with Wilson's disease had a significant (p < 0.05) increase in mean muscle copper concentration when compared with that of control subjects, there was too much overlap for this to be a useful diagnostic test. Ten of the 14 patients with Wilson's disease were estimated to have an increased amount of copper in their muscle tissues, averaging 4.5 mg. This amount of copper is small when compared with the excess copper in the liver and central nervous system of patients with Wilson's disease and suggests that the muscle is only a secondary site for deposition of copper.
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        • Tu J.B.
        • Blackwell R.Q.
        • Hou T.Y.
        Tissue copper levels in Chinese patients with Wilson's disease.
        Neurology. 1963; 13: 155
        • Fell G.S.
        • Smith H.
        • Howie R.A.
        Neutron activation analysis for copper in biological material applied to Wilson's disease.
        J. Clin. Path. 1968; 21: 8
        • Sternlieb I.
        • Scheinberg I.H.
        Prevention of Wilson's disease in asymptomatic patients.
        New Eng. J. Med. 1968; 278: 352
        • Smallwood R.A.
        • Williams H.A.
        • Rosenoer V.M.
        • Sherlock S.
        Liver-copper levels in liver disease. Studies using neutron activation analysis.
        Lancet. 1968; 2: 1310
        • Uzman L.L.
        Histochemical localization of copper with rubeanic acid.
        Lab. Invest. 1956; 5: 299
        • Kusaka Y.
        • Chen P-Y.
        • Tsuji H.
        • Imai S.
        Radioactivation analysis of trace elements in rice plant: Determinations of manganese, magnesium, sodium, potassium, chlorine, bromine, copper, and zinc.
        Radioisotopes. 1967; 16: 526
        • Moav B.
        The determination of manganese in urine by neutron activation analysis.
        Int. J. Appl. Radiat. 1965; 16: 365
        • Tipton I.H.
        • Cook M.J.
        Trace elements in human tissue. II. Adult subjects from the United States.
        Health Phys. 1963; 9: 103
        • Wilmer H.A.
        Changes in structural components of human body from 6 lunar months to maturity.
        in: Proc. Soc. Exp. Biol. Med. 43. 1940: 545
        • Osborn S.B.
        • Walshe J.M.
        Studies with radioactive copper (64Cu and 67Cu) in relation to the natural history of Wilson's disease.
        Lancet. 1967; 1: 346