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Research Article| Volume 76, ISSUE 3, P439-444, September 1970

Erythropoietin: A complex with different in vivo and in vitro activities

  • Peter P. Dukes
    Correspondence
    Reprint requests: Peter P. Dukes, Ph.D., Childrens Hospital of Los Angeles, P.O. Box 54700, Terminal Annex, Los Angeles, Calif. 90054.
    Affiliations
    From the Division of Hematology, The Childrens Hospital of Los Angeles, Los Angeles, Calif. U.S.A.

    From the Departments of Biochemistry and of Pediatrics, University of Southern California School of Medicine Los Angeles, Calif. U.S.A.
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  • Denman Hammond
    Affiliations
    From the Division of Hematology, The Childrens Hospital of Los Angeles, Los Angeles, Calif. U.S.A.

    From the Departments of Biochemistry and of Pediatrics, University of Southern California School of Medicine Los Angeles, Calif. U.S.A.
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  • Nomie A. Shore
    Affiliations
    From the Division of Hematology, The Childrens Hospital of Los Angeles, Los Angeles, Calif. U.S.A.

    From the Departments of Biochemistry and of Pediatrics, University of Southern California School of Medicine Los Angeles, Calif. U.S.A.
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  • Jorge A. Ortega
    Affiliations
    From the Division of Hematology, The Childrens Hospital of Los Angeles, Los Angeles, Calif. U.S.A.

    From the Departments of Biochemistry and of Pediatrics, University of Southern California School of Medicine Los Angeles, Calif. U.S.A.
    Search for articles by this author
DOI:https://doi.org/10.5555/uri:pii:0022214370900533
Erythropoietin: A complex with different in vivo and in vitro activities
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      Abstract

      Erythropoietin preparations exhibiting the same activity in the exhypoxic polycythemic mouse assay, which quantitates new red cell formation, differ from each other in their ability to stimulate heme synthesis and glucosamine incorporation in bone marrow cells in culture. This suggests that erythropoietin action may result from the separate stimulation by different factors of specific processes of erythroid differentiation.
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      References

        • Goldwasser E.
        Biochemical control of erythroid cell development.
        in: Current topics in developmental biology. vol. 1. Academic Press, Inc, New York1966: 173
        • Dukes P.P.
        Erythropoietin. Its nature and mechanism of action.
        in: Karlson P. Wirkungsmechanismen der Hormone, 18, Mosbacher Colloquium. Springer-Verlag, Berlin1967: 197
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        Erythropoietin and the regulation of erythropoiesis.
        University of Chicago Press, Chicago1970
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        Bio-assay of erythropoietin in mice made polycythaemic by exposure to air at reduced pressure.
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        • Dukes P.P.
        • Hammond D.
        • Shore N.A.
        Comparison of erythropoietin preparations yielding different dose-response slopes in the exhypoxic polycythemic mouse assay.
        J. Lab. Clin. Med. 1969; 74: 250
        • Dukes P.P.
        • Goldwasser E.
        On the mechanism of erythropoietin induced differentiation. III. The nature of erythropoietin action on (14C) glucosamine incorporation by marrow cells in culture.
        Biochim. Biophys. Acta. 1965; 108: 447
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        in: Wolstenholme G.E.W. O'Connor M. Ciba Foundation Symposium on haemopoiesis, cell production and its regulation. Little, Brown & Company, Boston1960: 397
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        Bull. W. H. O. 1966; 35: 751
        • Dukes P.P.
        Fractionation of human urinary erythropoietin preparations: Components stimulating different processes in erythroid differentiation.
        in: Fed. Proc. 29. 1970: 873 (Abst.)
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      Article info

      Publication history

      Accepted: June 27, 1970
      Received: April 15, 1970

      Footnotes

      ☆This investigation was supported by Grants HE 10880, AM 04048, and TI HD 48 from the National Institutes of Health, United States Public Health Service.

      Identification

      DOI: https://doi.org/10.5555/uri:pii:0022214370900533

      Copyright

      © 1970 Published by Elsevier Inc.

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