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Abstract
Transplanted hematopoietic stem cells seed in the spleen of lethally irradiated mice
where they differentiate and mature into discrete colonies of hematopoietic tissue.
In plethoric mice no erythroid colonies are formed. Those cells which usually supply
the erythron apparently become committed to the erythroid series and proliferate but
fail to mature into recognizable erythroid elements unless exogenous erythropoietin
is administered. The extent and limits of the proliferation were investigated by transplanting
bone marrow cells into plethoric mice, allowing the cells to multiply for 4 to 7 days
in the absence of erythropoietin, and then stimulating their maturation with exogenous
erythropoietin. Since the duration of erythropoietin stimulation was constant, variation
in the size of mature erythroid colonies presumably reflects variation of the size
of the colonies of erythroid-committed cells before erythropoietin was administered.
The colonies of cells, unrecognizable but apparently committed to the erythron, increased
in size through the seventh day post transplantation, remained constant in size for
24 hours, and then appeared to decrease. It would appear that these cells were able
to undergo several divisions in the absence of erythropoietin, but their growth potential
is under some constraint and limitation.
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References
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Article info
Publication history
Accepted:
July 8,
1970
Received:
January 29,
1970
Identification
Copyright
© 1970 Published by Elsevier Inc.
