Research Article| Volume 76, ISSUE 3, P445-450, September 1970

Proliferation of erythroid-committed cells in the absence of erythropoietin

  • Lois F. O'Grady
    Reprint requests: Lois O'Grady, M.D., Assistant Professor of Medicine, School of Medicine, Davis, Calif. 95616.
    From the Section of Hematology, Department of Internal Medicine, University of California School of Medicine Davis, Calif. U.S.A.
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  • Jerry P. Lewis
    From the Section of Hematology, Department of Internal Medicine, University of California School of Medicine Davis, Calif. U.S.A.
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      Transplanted hematopoietic stem cells seed in the spleen of lethally irradiated mice where they differentiate and mature into discrete colonies of hematopoietic tissue. In plethoric mice no erythroid colonies are formed. Those cells which usually supply the erythron apparently become committed to the erythroid series and proliferate but fail to mature into recognizable erythroid elements unless exogenous erythropoietin is administered. The extent and limits of the proliferation were investigated by transplanting bone marrow cells into plethoric mice, allowing the cells to multiply for 4 to 7 days in the absence of erythropoietin, and then stimulating their maturation with exogenous erythropoietin. Since the duration of erythropoietin stimulation was constant, variation in the size of mature erythroid colonies presumably reflects variation of the size of the colonies of erythroid-committed cells before erythropoietin was administered. The colonies of cells, unrecognizable but apparently committed to the erythron, increased in size through the seventh day post transplantation, remained constant in size for 24 hours, and then appeared to decrease. It would appear that these cells were able to undergo several divisions in the absence of erythropoietin, but their growth potential is under some constraint and limitation.
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        • O'Grady L.F.
        • Lewis J.P.
        • Lange R.D.
        • Trobaugh Jr., F.E.
        Effect of erythropoietin on transplanted hematopoietic tissue.
        Amer. J. Physiol. 1968; 215: 176
        • O'Grady L.F.
        • Lewis J.P.
        • Trobaugh Jr., F.E.
        The effect of erythropoietin on differentiated erythroid precursors.
        J. Lab. Clin. Med. 1968; 71: 693
        • Bleiberg I.
        • Liron M.
        • Feldman M.
        Reversion by erythropoietin of the suppression of erythroid clones caused by transfusion induced polycythemia.
        Transplantation. 1965; 3: 706
        • Bleiberg I.
        • Liron M.
        • Feldman M.
        Studies on the regulation of hematopoietic spleen colonies.
        Blood. 1967; 28: 469
        • O'Grady L.F.
        • Lewis J.P.
        • Trobaugh Jr., F.E.
        Effect of timed doses of erythropoietin on the development of hematopoietic tissue.
        Exp. Hemat. 1967; 12: 62
        • Curry J.L.
        • Trentin J.J.
        • Wolf N.
        Hematopoietic spleen colony studies. II. Erythropoiesis.
        J. Exp. Med. 1967; 125: 703
        • Lewis J.P.
        • Passovoy M.
        • McFate P.A.
        • Trobaugh Jr., F.E.
        The rate of growth and pattern of differentation of transplanted thawed cells.
        Transplantation. 1967; 5: 435
        • Filmanowicz E.
        • Gurney C.W.
        Studies on erythropoiesis. XVI. Response to a single dose of erythropoietin in polycythemic mouse.
        J. Lab. Clin. Med. 1961; 57: 65
        • Alpen E.L.
        • Cranmore D.
        Observations on the regulation of erythropoiesis on cellular dynamics by Fe59 autoradiography.
        in: Stohlman Jr., F. The kinetics of cellular proliferation. Grune & Stratton, Inc, New York1959: 290
        • Blackett N.M.
        • Roylance P.J.
        • Adams K.
        Studies of the capacity of bone marrow cells to restore erythropoiesis in heavily irradiated rats.
        Brit. J. Haemat. 1964; 10: 453
        • Bruce W.R.
        • McCulloch E.A.
        The effect of erythropoietic stimulation on the hematopoietic colony forming cells of mice.
        Blood. 1964; 23: 216
        • Kubanek B.
        • Ferrari L.
        • Tyler W.S.
        • Howard D.
        • Jay S.
        • Stohlman Jr., F.
        Regulation of erythropoiesis. XXIII. Dissociation between stem cell and erythroid response to hypoxia.
        Blood. 1968; 32: 586
        • Stohlman Jr., F.
        • Ebbe S.
        • Morse B.
        • Howard D.
        • Donovan J.
        Regulation of erythropoiesis. XX. Kinetics of red cell production.
        Ann. N. Y. Acad. Sci. 1968; 149: 156
        • Hanna I.R.A.
        Response of early precursors to bleeding.
        Nature. 1967; 204: 355
        • Reissmann K.R.
        • Samorapoompichit S.
        Effect of erythropoietin on regeneration of hematopoietic stem cells after 5-fluorouracil administration.
        J. Lab. Clin. Med. 1969; 73: 544
        • DeGowin R.L.
        • Johnson S.
        Development of response to erythropoietin and repletion of the stem cell compartment after irradiation.
        J. Lab. Clin. Med. 1968; 72: 893