Research Article| Volume 76, ISSUE 3, P466-471, September 1970

Detection of an inhibitor of hemoglobin synthesis in rat plasma

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      The presence of an inhibitor of hemoglobin synthesis has been detected in rat plasma and serum. The inhibitor decreased the uptake of 59Fe and incorporation of glycine-2-14C into rat reticulocytes harvested from phenylhydrazine-treated rats. The inhibition was dose related above plasma volumes of 0.5 ml., the inhibitor activity being threefold greater in serum from animals with phenylhydrazine-induced anemia than that in normal serum. The factor was nondialyzable, was precipitated with concentrations of ammonium sulfate above 40 per cent, and was extractable into butanone-2. The inhibition was shown not to be due to heme.
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