This paper is only available as a PDF. To read, Please Download here.
Abstract
An evaluation was made of methods in which the colorimetric total iron-binding capacity
(TIBC) or isotopic unsaturated iron-binding capacity (UIBC) is determined after removing
the unbound excess of added iron with either an ion exchange resin, light MgCO3, or hemoglobin-coated charcoal. The colorimetric and isotopic assays were compared
by performing them simultaneously with light MgCO3; isotopic determinations alone were performed with resin and coated charcoal to compare
the efficacy of the 3 adsorbents. The resin method was more variable and less accurate
than the other techniques. The presence of hemoglobin, ferritin, or iron dextran did
not affect isotopic measurements, but the latter 2 contaminants interfered markedly
with colorimetric determinations of plasma iron or TIBC. With all the adsorbents,
there were systematic errors due to incomplete removal of unbound iron excess and
to adsorption of plasma water with concentration of supernatant protein. Although
these errors are of little importance in routine clinical determinations, their evaluation
provides a useful monitor for the day-to-day performance of the adsorption techniques.
It is concluded that light MgCO3 and coated charcoal are equally valid for determining the iron-binding capacity;
however, light MgCO3 is preferable because it is readily available, requires no preparation, and can be
used interchangeably in isotopic and colorimetric assays.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Translational ResearchAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Bound iron and unsaturated iron-binding capacity of serum. Rapid and reliable quantitative determination.in: ed. 6. Proc. Soc. Exp. Biol. Med. 87. 1954: 443
- Serum unsaturated iron-binding capacity.Amer. J. Clin. Path. 1958; 30: 87
- Direct determination and binding capacity of serum iron.Clin. Chem. 1961; 7: 241
- Improved rapid methods for the determination of iron content and binding capacity of serum.J. Clin. Path. 1962; 15: 156
- Serum iron and iron-binding capacity in normal subjects.Canad. Med. Ass. J. 1964; 90: 618
- Direct measurement of serum iron and binding capacity.Clin. Chem. 1966; 12: 47
- A new method for the determination of serum iron-binding capacity. I.J. Lab. Clin. Med. 1956; 48: 274
- On the determination of serum iron and iron-binding capacity.Clin. Chim. Acta. 1958; 3: 523
- Serum iron and iron-binding capacity in clinical diagnosis.Clin. Chem. 1959; 5: 1
- Determination of the serum iron-binding capacity.J. Clin. Path. 1965; 18: 453
- Studies on the serum iron-binding capacity.J. Clin. Path. 1965; 18: 446
- The determination of the unsaturated iron-binding capacity of serum using radioactive iron.S. Afr. J. Med. Sci. 1959; 24: 93
- A rapid radioactive method for the determination of the serum iron-binding capacity.Amer. J. Clin. Path. 1961; 35: 403
- A note on the measurement of the unsaturated iron-binding capacity of serum using radio-active iron.S. Afr. J. Med. Sci. 1965; 30: 71
- Comparison of light magnesium carbonate and amberlite sponge adsorbants in the measurement of the latent iron-binding capacity of serum.Amer. J. Clin. Path. 1965; 44: 77
- Comparison of methods designed to measure transferrin and iron-binding capacity of serum.Amer. J. Clin. Path. 1967; 47: 326
- The determination of the total iron-binding capacity of serum.Clin. Chim. Acta. 1957; 2: 221
- Plasma iron and iron-binding capacity levels in health and disease: with an improved method for the estimation of plasma iron concentration and total iron-binding capacity.Aust. Ann. Med. 1960; 9: 209
- Macro and micro methods for the determination of serum iron and iron-binding capacity.Clin. Chem. 1963; 9: 188
- A simple serum iron method using the new sensitive chromogen tripyridyl-s-triazine.Clin. Chem. 1964; 10: 21
- Use of protein solubilizing detergent for rapid serum iron and iron binding capacity assays.Amer. J. Med. Techn. 1965; 31: 403
- Method for the automatic determination of serum iron.J. Clin. Path. 1965; 18: 98
- A comparison of the iron-binding capacity of the serum studied by means of biochemical and isotope methods.Pol. Med. J. 1966; 5: 286
- Hayes R.L. Goswitz F.A. Murphy B.E.P. Radioisotopes in medicine: in vitro studies. United States Atomic Energy Commission, Oak Ridge, Tenn1968: 351-363
- Method for the determination of unsaturated iron-binding capacity of serum using radioactive iron and magnesium carbonate.J. Clin. Path. 1968; 21: 183
- Coated charcoal assay of unsaturated iron-binding capacity.J. Lab. Clin. Med. 1966; 67: 855
- Standard deviation. A practical means for the measurement and control of the precision of clinical laboratory determinations.Amer. J. Clin. Path. 1957; 27: 551
- Statistical methods.in: ed. 6. Iowa State University Press, Ames, Iowa1967: 92
- ed. 6. Statistical methods. Iowa State University Press, Ames, Iowa1967: 116
- Einfache mikro-kolorimetrische Eisenbestimmungsmethode.Biochem. Z. 1927; 181: 391
- Iron metabolism.in: Little, Brown & Company, Boston1962: 18
- The determination of iron in plasma or serum.Biochem. J. 1955; 59: 599
- Measurement of serum vitamin B12 level using radioisotope dilution and coated charcoal.Blood. 1965; 26: 202
Article info
Publication history
Accepted:
June 17,
1970
Received:
January 8,
1970
Footnotes
☆This investigation was supported by United States Public Health Service Research Grant 5-RO1-HE-06242, Training Grants T1-AM-5130 and AM-06742, and the World Health Organization Program of Iron Standardization.
Identification
Copyright
© 1970 Published by Elsevier Inc.
