Research Article| Volume 76, ISSUE 3, P523-529, September 1970

A simple and rapid dye test for glucose-6-phosphate dehydrogenase deficiency for routine use

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      The methylene blue uptake test for glucose-6-phosphate dehydrogenase (G-6-PD) deficiency has been evaluated as a screening test in 1,432 male Negro patients. The incidence of positive results was 9.5 per cent with only 6 false positive values. Further study with this test resulted in the development of an even simpler modification. Capable of processing 150 to 200 samples per hour, this test has been used to survey 8,156 hospital patients on a routine basis without regard to ethnic origin. Approximately 200 cases of G-6-PD deficiency were recognized. All but 19 were confirmed by direct spectrophotometric assay. No false negative results were encountered with either technique in a random selection of 300 negative samples. The test can be used for individual samples, but its speed and reliability make it exceptionally suitable for use as a routine procedure for all hospital admissions, for screening and identification of G-6-PD-deficient blood donors as well as for other large groups. This is of special significance in the case of military personnel subject to prophylactic treatment with antimalarial drugs.
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        • Beutler E.
        The hemolytic effects of primaquine and related compounds: A review.
        Blood. 1959; 14: 103
        • Kornberg A.
        • Horecker B.L.
        Glucose-6-phosphate dehydrogenase.
        in: Methods in enzymology. vol. 1. Academic Press, Inc, New York1955: 323
        • Beutler E.
        A series of new screening procedures for pyruvate kinase deficiency, glucose-6-phosphate dehydrogenase deficiency and glutathione reductase deficiency.
        Blood. 1966; 28: 553
        • Fairbanks V.F.
        • Fernandez M.N.
        The identification of metabolic errors associated with hemolytic anemia.
        J. A. M. A. 1969; 208: 316
        • Sass M.D.
        • Caruso C.J.
        • Axelrod D.R.
        Rapid screening for D-glucose-6-phosphate: NADP oxidoreductase deficiency with methylene blue.
        J. Lab. Clin. Med. 1966; 68: 156
        • Sass M.D.
        • Caruso C.J.
        • Axelrod D.R.
        Accumulation of methylene blue by metabolizing erythrocytes.
        J. Lab. Clin. Med. 1967; 69: 447
        • Sass M.D.
        • Caruso C.J.
        • Farhangi M.
        TPNH-methemoglobin reductase deficiency: A new red-cell enzyme defect.
        J. Lab. Clin. Med. 1967; 70: 760
        • Carson P.E.
        • Frischer H.
        Glucose-6-phosphate dehydrogenase deficiency and related disorders of the pentose phosphate pathway.
        Amer. J. Med. 1966; 41: 744
        • Theil G.B.
        • Brodine C.E.
        • Doolan P.D.
        Red cell glutathione content and stability in renal insufficiency.
        J. Lab. Clin. Med. 1961; 58: 736
        • Herz F.
        • Kaplan E.
        • Scheye E.
        Diagnosis of erythrocyte glucose-6-phosphate dehydrogenase deficiency in the Negro male despite hemolytic crisis.
        Blood. 1970; 35: 90
        • Sass M.D.
        • Caruso C.J.
        • Axelrod D.R.
        Evaluation of stored blood viability by methylene blue reduction.
        J. Lab. Clin. Med. 1969; 73: 744
        • Bowman J.E.
        • Carson P.E.
        • Frischer H.
        • Kahn M.
        • Ajmar F.A.
        A capillary tube, Nile Blue methemoglobin reduction test for glucose-6-phosphate dehydrogenase deficiency.
        in: Proc. 10th Congr. Int. Soc. Blood Transfus, Stockholm, 19641965: 592
        • Mentzner Jr., W.C.
        • Lubin B.H.
        • Nathan D.G.
        Screening for sickle-cell trait and G-6-PD deficiency.
        New Eng. J. Med. 1970; 282: 1155