This paper is only available as a PDF. To read, Please Download here.
Abstract
The methylene blue uptake test for glucose-6-phosphate dehydrogenase (G-6-PD) deficiency
has been evaluated as a screening test in 1,432 male Negro patients. The incidence
of positive results was 9.5 per cent with only 6 false positive values. Further study
with this test resulted in the development of an even simpler modification. Capable
of processing 150 to 200 samples per hour, this test has been used to survey 8,156
hospital patients on a routine basis without regard to ethnic origin. Approximately
200 cases of G-6-PD deficiency were recognized. All but 19 were confirmed by direct
spectrophotometric assay. No false negative results were encountered with either technique
in a random selection of 300 negative samples. The test can be used for individual
samples, but its speed and reliability make it exceptionally suitable for use as a
routine procedure for all hospital admissions, for screening and identification of
G-6-PD-deficient blood donors as well as for other large groups. This is of special
significance in the case of military personnel subject to prophylactic treatment with
antimalarial drugs.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Translational ResearchAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- The hemolytic effects of primaquine and related compounds: A review.Blood. 1959; 14: 103
- Glucose-6-phosphate dehydrogenase.in: Methods in enzymology. vol. 1. Academic Press, Inc, New York1955: 323
- A series of new screening procedures for pyruvate kinase deficiency, glucose-6-phosphate dehydrogenase deficiency and glutathione reductase deficiency.Blood. 1966; 28: 553
- The identification of metabolic errors associated with hemolytic anemia.J. A. M. A. 1969; 208: 316
- Rapid screening for D-glucose-6-phosphate: NADP oxidoreductase deficiency with methylene blue.J. Lab. Clin. Med. 1966; 68: 156
- Accumulation of methylene blue by metabolizing erythrocytes.J. Lab. Clin. Med. 1967; 69: 447
- TPNH-methemoglobin reductase deficiency: A new red-cell enzyme defect.J. Lab. Clin. Med. 1967; 70: 760
- Glucose-6-phosphate dehydrogenase deficiency and related disorders of the pentose phosphate pathway.Amer. J. Med. 1966; 41: 744
- Red cell glutathione content and stability in renal insufficiency.J. Lab. Clin. Med. 1961; 58: 736
- Diagnosis of erythrocyte glucose-6-phosphate dehydrogenase deficiency in the Negro male despite hemolytic crisis.Blood. 1970; 35: 90
- Evaluation of stored blood viability by methylene blue reduction.J. Lab. Clin. Med. 1969; 73: 744
- A capillary tube, Nile Blue methemoglobin reduction test for glucose-6-phosphate dehydrogenase deficiency.in: Proc. 10th Congr. Int. Soc. Blood Transfus, Stockholm, 19641965: 592
- Screening for sickle-cell trait and G-6-PD deficiency.New Eng. J. Med. 1970; 282: 1155
Article info
Publication history
Accepted:
July 5,
1970
Received:
April 9,
1970
Identification
Copyright
© 1970 Published by Elsevier Inc.
