The measurement of testosterone in male plasma by competitive protein binding

J.P. Liberti; C.H. Duvall; M.A. Mackler; G.R. Prout

doi:10.5555/uri:pii:0022214370900648

Research Article| Volume 76, ISSUE 3, P530-536, September 1970

The measurement of testosterone in male plasma by competitive protein binding

J.P. Liberti
J.P. Liberti
Correspondence
Address reprint requests to Dr. Liberti at the address below. Address all correspondence to J. P. Liberti, Department of Biochemistry, Medical College of Virginia, Health Sciences Division, Virginia Commonwealth University, Richmond, Va. 23219.
Affiliations
From the Department of Biochemistry and Division of Urology, Medical College of Virginia, Health Sciences Division of Virginia Commonwealth University Richmond, Va. U.S.A.
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C.H. Duvall
C.H. Duvall
Affiliations
From the Department of Biochemistry and Division of Urology, Medical College of Virginia, Health Sciences Division of Virginia Commonwealth University Richmond, Va. U.S.A.
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M.A. Mackler
M.A. Mackler
Footnotes
Affiliations
From the Department of Biochemistry and Division of Urology, Medical College of Virginia, Health Sciences Division of Virginia Commonwealth University Richmond, Va. U.S.A.
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G.R. Prout Jr.
G.R. Prout Jr.
Footnotes
Affiliations
From the Department of Biochemistry and Division of Urology, Medical College of Virginia, Health Sciences Division of Virginia Commonwealth University Richmond, Va. U.S.A.
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Author Footnotes
∗∗ Fellow, American Cancer Society.
∗∗∗ Present address: G. R. Prout, Jr., Department of Urology, Massachusetts General Hospital, Boston, Mass.

DOI:https://doi.org/10.5555/uri:pii:0022214370900648

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Abstract

An accurate and reproducible procedure is described for the determination of testosterone from male plasma with the use of competitive protein binding. One milliliter of plasma is required to satisfactorily measure testosterone in plasma from normal orchiectomized men. The mean recovery of testosterone in approximately 300 samples was 60 ± 10 per cent (S.D.). The mean plasma testosterone level in 50 normal men was 5.6 ± 2.3 mμg per milliliter (S.D.). In 30 patients with carcinoma of the prostate, the mean was 7.9 ± 2.2 mμg per milliliter (S.D.) which decreased to 0.6 mμg per milliliter after orchiectomy.

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Article info

Publication history

Accepted: July 9, 1970

Received: February 16, 1970

Footnotes

☆This work was supported by United States Public Health Service Grant CA 06462.

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DOI: https://doi.org/10.5555/uri:pii:0022214370900648

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The measurement of testosterone in male plasma by competitive protein binding

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