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Abstract
When erythrocyte membranes are incubated with hemolysates in the presence of adenosine
triphosphate (ATP), they release adenosine diphosphate (ADP). This effect is ouabain
sensitive and is attributable to membrane adenosine triphosphatase (ATPase). The released
ADP stimulates lactate production from fructose diphosphate, and this effect persists
in the hemolysate after the hemolysate is cleared of membranes. Membranes have no
effect on lactate production from glucose in hemolysates, presumably because the utilization
of this substrate creates ADP. The data suggest that the membrane cation pump is linked
to hemolysate glycolysis through the production of ADP by ATPase and that the ADP
produced by membrane ATPase contributes to the requirements for ADP in the cytosome.
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Article info
Publication history
Accepted:
May 25,
1970
Received:
January 5,
1970
Footnotes
☆This research was supported by United States Public Health Service Grants HD-02777 and 1-TO1-AM05581 and by a grant from the John A. Hartford Foundation.
Identification
Copyright
© 1970 Published by Elsevier Inc.
