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When erythrocyte membranes are incubated with hemolysates in the presence of adenosine triphosphate (ATP), they release adenosine diphosphate (ADP). This effect is ouabain sensitive and is attributable to membrane adenosine triphosphatase (ATPase). The released ADP stimulates lactate production from fructose diphosphate, and this effect persists in the hemolysate after the hemolysate is cleared of membranes. Membranes have no effect on lactate production from glucose in hemolysates, presumably because the utilization of this substrate creates ADP. The data suggest that the membrane cation pump is linked to hemolysate glycolysis through the production of ADP by ATPase and that the ADP produced by membrane ATPase contributes to the requirements for ADP in the cytosome.
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Accepted: May 25, 1970
Received: January 5, 1970
☆This research was supported by United States Public Health Service Grants HD-02777 and 1-TO1-AM05581 and by a grant from the John A. Hartford Foundation.
© 1970 Published by Elsevier Inc.