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Abstract
In 52 dogs anesthetized with sodium pentobarbital, the septal artery and the atrioventricular
(A-V) node artery were cannulated for direct perfusion of the His bundle and A-V node.
Advantages of this method include preservation of the heart in situ with its normal
innervation intact and the production of localized effects by injection of small (2
ml.) amounts of test substances. One disadvantage is the unavoidable simultaneous
perfusion of ventricular myocardium. Utilizing this method, we produced transient
A-V block with acetylcholine, 0.1 to 1.0 μg per milliliter, and prolonged block with
neostigmine, 10 μg per milliliter; this effect is reversed and blocked with local
administration of atropine, 10 μg per milliliter. Direct perfusion of the septal artery
or the A-V-node artery with norepinephrine or isoproterenol, 0.1 μg per milliliter,
produced an A-V junctional tachycardia which could be blocked with propranolol, 10
μg per milliliter; more prolonged tachycardia was produced with tyramine, 10 μg per
milliliter, or guanethidine, 100 μg per milliliter, and could be transiently reversed
with acetylcholine, which also caused transient simultaneous A-V block. These experiments
demonstrate the specific production and precise control of transient or prolonged
A-V block or junctional tachycardia in the intact heart in situ.
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Article info
Publication history
Accepted:
May 25,
1970
Received:
February 9,
1970
Footnotes
☆This work was supported in part by grants from the United States Public Health Service (H-5197, H-7108, HE 11,310, and PH 43-67-1441), the Michigan Heart Association, and the Alabama Heart Association.
Identification
Copyright
© 1970 Published by Elsevier Inc.