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Research Article| Volume 76, ISSUE 2, P333-337, August 1970

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Immunoglobulin synthesis by blood lymphocytes—Method of quantitation and the effect of phytohemagglutinin

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      Abstract

      Reproducible quantitation of cell-associated immunoglobulins was accomplished with the use of an ammonium sulfate technique. Blood lymphocytes produced immunoglobulins when cultured in a protein-containing medium; the quantity synthesized was similar in replicate cultures from the same donor but varied from donor to donor. In no instance was phytohemagglutinin blastic transformation associated with an increase in immunoglobulin production above that seen in parallel cultures ontaining no phytohemagglutinin.
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