This paper is only available as a PDF. To read, Please Download here.
Reproducible quantitation of cell-associated immunoglobulins was accomplished with the use of an ammonium sulfate technique. Blood lymphocytes produced immunoglobulins when cultured in a protein-containing medium; the quantity synthesized was similar in replicate cultures from the same donor but varied from donor to donor. In no instance was phytohemagglutinin blastic transformation associated with an increase in immunoglobulin production above that seen in parallel cultures ontaining no phytohemagglutinin.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Translational Research
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- The formation of immunoglobulins by human tissues in vitro. I. The methods and their specificity.Immunology. 1966; 11: 1
- Studies on human peripheral blood lymphocytes in vitro. V. Biosynthesis of immunoglobulins.J. Immun. 1968; 101: 458
- Gamma globulin production by human lymphocytes in vitro.Exp. Cell Res. 1963; 32: 592
- The production of immunoglobulins by human peripheral blood lymphocytes in vitro.Clin. Exp. Immun. 1967; 2: 377
- The in vitro production of antibody by lymphocytes.Lancet. 1963; 1: 1292
- Studies on rabbit lymphocytes in vitro. III. Protein, RNA, and DNA synthesis by lymphocyte cultures after stimulation with staphylococcal filtrate, with antiallotype serum, and with heterologous antiserum to rabbit whole serum.J. Exp. Med. 1965; 122: 823
- Protein synthesis in phytohemagglutinin-stimulated cultures of human lymphocytes.in: Fed. Proc. 25. 1966: 439
- The formation of immunoglobulins by circulating lymphocytes.in: Seminars Hemat. 6. 1969: 84
- The effect of phytohemagglutinin and lymphocyte mitogens on immunoglobulin synthesis by human peripheral blood lymphocytes in vitro.Clin. Exp. Immun. 1968; 3: 393
- A quantitative immunochemical measure of the primary interaction between I-BSA and antibody.J. Infect. Dis. 1958; 103: 239
- The measurement of immunoglobulins associated with human peripheral lymphocytes.J. Immun. 1970; 104: 367
- The immunologic specificity of the primary interaction between 125I-Fab and anti-F(ab)2.in: Fed. Proc. 28. 1969: 364
- Quantitation of the suppressive effect of phytohemagglutinin on Ig synthesis by human blood lymphocytes.Arthritis Rheum. 1969; 12: 698
- A new method for preparing buffy coat-poor blood.Transfusion. 1962; 2: 221
- Comparative response of normal human thymus and lymph node cells to phytohemagglutinin in culture.Blood. 1967; 29: 594
- Studies on rabbit lymphocytes in vitro. I. Stimulation of blast transformation with antiallotype serum.J. Exp. Med. 1965; 122: 423
Accepted: June 9, 1970
Received: February 2, 1970
☆This work was supported by United States Public Health Service Grants CA-11800-01 and AI-2E-214.
© 1970 Published by Elsevier Inc.