Research Article| Volume 76, ISSUE 2, P338-348, August 1970

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The fluorometric measurement of 4,4′-diaminodiphenyl sulfone and its acetylated derivatives in plasma and urine

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      A fluorometric method for the concurrent determination of DDS, MADDS, and DADDS in biological fluids has been described. Various tests have been applied that testify to the validity of the method. Lower limits of variability were 0.004 μg, 0.002 μg, and 0.2 μg of DDS, MADDS, and DADDS, respectively. The practical limits of sensitivity were 0.01 μg of DDS and MADDS with the use of 1 ml. of original sample. High sensitivity for measurement of DADDS was not pursued since no evidence was obtained that this compound was a metabolite of DDS or MADDS in animals or man. The specificity of the method has been demonstrated by examination of the contribution of various sulfone derivatives known, or suspected, to be metabolites of DDS in biological systems. Procedures for measuring various conjugates of DDS in urine have been described.


      DDS (4,4′-diaminodiphenyl sulfone), MADDS (4-amino-4′-acetamidodiphenyl sulfone), DADDS (4,4′-diacetamidodiphenyl sulfone), DDS-G (4-amino-4′-N-glucosiduronodiphenyl sulfone), DDS-S (4-amino-4′-sulfamatodiphenyl sulfone), DDS-diS (4,4′-disulfamatodiphenyl sulfone), MADDS-S (4-acetamino-4′-sulfamatodiphenyl sulfone), DDS-H (4-amino-4′-hydroxyaminodiphenyl sulfone), MADDS-H (4-acetamido-4′-hydroxyaminodiphenyl sulfone), DDS-diH (4,4′-dihydroxyaminodiphenyl sulfone), DDS-3-OH (3-hydroxy-4,4′-diaminodiphenyl sulfone), MADDS-3′-OH (3-hydroxy-4-amino-4′-acetamidodiphenyl sulfone), DDS-2-OH (2-hydroxy-4,4′-diaminodiphenyl sulfone), MADDS-2′-OH (2-hydroxy-4-amino-4′-acetamidodiphenyl sulfone), DDS-2,2′-diOH (2,2′-dihydroxy-4,4′-diaminodiphenyl sulfone)
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