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Abstract
It has been demonstrated that clearance of 2 strains of Pseudomonas aeruginosa from lungs of normal mice differs from the pattern observed with other gramnegative
bacilli. The purpose of this study was to examine the pulmonary clearance of several
P. aeruginosa strains and to identify factors responsible for the altered clearance pattern. Mice
were nebulized 30 min. via a Henderson aerosol apparatus with 14 P. aeruginosa strains. Three clearance patterns were seen: (1) multiplication of organisms in lungs
for one hour after nebulization, followed by progressive clearance; (2) relatively
little change in bacterial count of the lungs for 4 hours after nebulization; and
(3) progressive clearance. Clearance patterns were not significantly correlated with
intraperitoneal LD50, phage, or pyocine type, Hab's “O” serotype, extracellular production of elastase,
hemolysin, or protease of the respective pseudomonas strains, or with the histologic
picture produced in the lungs. Organisms producing type 1 clearance pattern produced
significantly greater amounts of extracellular lecithinase than did other strains.
This suggests that alteration of pulmonary surfactant by lecithinase may enable early
multiplication of some Pseudomonas strains in lungs, predisposing to pneumonia. Other
differences in histologic changes in lungs may be related to yet additional extracellular
enzymes of P. aeruginosa.
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Article info
Publication history
Accepted:
July 13,
1970
Received:
April 20,
1970
Footnotes
☆This research was supported in part by United States Public Health Service Training Grant 5 TO1 AI 00030 and Research Grant AI 08664 from the National Institute of Allergy and Infectious Diseases, and by United States Public Health Service Grant 5 RO1 CC 00202 from the National Communicable Disease Center, Atlanta, Ga.
☆☆Presented in part at the national meeting of the American Federation for Clinical Research, May 3, 1969, Atlantic City, N. J.
Identification
Copyright
© 1970 Published by Elsevier Inc.
