This paper is only available as a PDF. To read, Please Download here.
Implantation of irradiated mouse spleens into the peritoneal cavity of mice did not alter the number of hematopoietic stem cells in the femoral marrow when assayed by the spleen colony method. On the other hand, there was a significantly greater number of hematopoietic stem cells in femoral marrow of spleen-implanted mice as compared to those in saline-treated mice 4 days after exposure to 200 r of x-irradiation (given 2 hours after spleen implants). Injections of homogenates made from spleens and of a water-soluble fraction of this homogenate similarly resulted in an increase in the rate of hematopoietic stem cell regeneration following 200 r. We postulate that a noncellular substance of splenic origin can increase stem cell proliferation in mice. Response to this stimulus is inhibited by the presence of a dense population of cells in the environment.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Translational Research
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- The effect of cyclophosphamide on hematopoietic stem cells.Radiat. Res. 1968; 36: 521
- The regulation of numbers of primitive hematopoietic cells.in: ed. 4. Proc. Nat. Acad. Sci. 54. 1965: 1148
- Abscopal effects on bone marrow stem cell kinetics.Exp. Hemat. 1969; 18: 15
- A direct measurement of the radiation sensitivity of normal mouse bone marrow cells.Radiat. Res. 1961; 14: 213
- Studies of hematopoiesis in murine spleens: significance of hematopoietic colonies formed on the surface of spleens.Cell Tissue Kinet. 1968; 1: 101
- The effect of cooling regimens on the transplantation potential of marrow.Transfusion. 1967; 7: 17
- The effect of a cell-derived factor and depleted cell population upon control of hematopoietic stem cells.Blood. 1969; 34: 836
- Comparison of the sensitivity of hematopoietic colony-forming cells in different proliferative states to vinblastine.J. Nat. Cancer Inst. 1967; 38: 393
- Cell division and cancer.Science. 1965; 149: 34
- Factors influencing hematopoietic spleen colony formation in irradiated mice. V. Effect of foreign plasma upon colony forming cell kinetics.J. Cell Physiol. 1968; 71: 227
- Colony formation on spleens of irradiated mice injected with human marrow cells.Exp. Hemat. 1967; 13: 22
- Erythropoietic colony formation in spleens of supralethally irradiated C3H mice following injection of human lymphocytes and bone marrow.Exp. Hemat. 1964; 7: 84
- Further studies on the effect of serum alpha marcroglobulin on regeneration of hematopoietic tissue after x-irradiation.Radiat. Res. 1968; 35: 378
- Stimulation of thymic and bone marrow regeneration in irradiated mice by protein fractions of human serum and sheep spleen.Radiat. Res. 1969; 40: 193
- Properties of the colony stimulating factor in leukemic and normal mouse serum.Aust. J. Exp. Biol. Med. Sci. 1968; 46: 715
- Early-onset diabetes mellitus in the general and Down's syndrome populations.Lancet. 1969; 1: 554
Accepted: July 16, 1970
Received: February 24, 1970With the technical assistance of MAX DANSBIE and S. ARTHUR CONTI
☆Studies reported here were supported by United States Public Health Service Grants CA04144-12 and AM12936-02 and grants from the Leukemia Research Foundation and the American Cancer Society.
© 1970 Published by Elsevier Inc.