Research Article| Volume 76, ISSUE 4, P584-592, October 1970

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Effect of a noncellular spleen-derived factor on recovery of hematopoietic stem cells from irradiation

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      Implantation of irradiated mouse spleens into the peritoneal cavity of mice did not alter the number of hematopoietic stem cells in the femoral marrow when assayed by the spleen colony method. On the other hand, there was a significantly greater number of hematopoietic stem cells in femoral marrow of spleen-implanted mice as compared to those in saline-treated mice 4 days after exposure to 200 r of x-irradiation (given 2 hours after spleen implants). Injections of homogenates made from spleens and of a water-soluble fraction of this homogenate similarly resulted in an increase in the rate of hematopoietic stem cell regeneration following 200 r. We postulate that a noncellular substance of splenic origin can increase stem cell proliferation in mice. Response to this stimulus is inhibited by the presence of a dense population of cells in the environment.
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