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Cornely's modification of the Boyden Chamber technique was used to detect the presence of chemotactic activity. Chemotactic activity was found in the synovial fluid 60 to 90 min. after injection of synthetic monosodium urate crystals in the canine joint. In comparison, no chemotactic activity was present in a saline-injected knee. Treatment of animals with colchicine suppressed the generation of chemotactic activity. Further studies will be necessary to determine whether these findings have applicability to the understanding of human gouty arthritis.
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Accepted: July 24, 1970
Received: November 15, 1969
☆Supported by National Institutes of Health Grants AM-12593 and FR-107, and a Grant from the Eastern Pennsylvania Chapter of the Arthritis Foundation.
☆☆Presented in part at the Annual Meeting of the American Rheumatism Association, Boston, Mass., June, 1969.
© 1970 Published by Elsevier Inc.